Exploring future applications of the apiculate yeast Hanseniaspora

Figures & data

Figure 1. Species belonging to the genus Hanseniaspora. These lemon-shaped yeasts are prevalent on ripe fruits and have been isolated from a diverse range of environments. Some of these apiculate species are associated with grapes and wine; grains and beer; agave plants and tequila; and cacao and coffee beans; as well as apple and pear cider.

Figure 2. A phylogenetic tree of members of the genus Hanseniaspora. Using maximum-likelihood analysis of the internal transcribed sequences of one representative of each Hanseniaspora species divides the genus into two large clades, a fast-evolving fruit clade and a slow-evolving fermentation clade. MEGA 11 software was used to draw the tree [218].

Figure 3. Effect of Hanseniaspora-initiated cultures in mixed-culture wine fermentations. Increases and decreases in metabolite concentrations are shown relative to the levels of those metabolites produced in fermentations conducted with a pure culture of Saccharomyces cerevisiae. References for Hanseniaspora uvarum [67,76,85,104,219–224], Hanseniaspora guilliermondii [31,220,221,225], Hanseniaspora vineae [68,69,75,79,85,105,226] and other Hanseniaspora species [75,223,227–229].

Figure 4. Biosynthesis pathways of fermentation-derived aroma compounds produced by grape-related Hanseniaspora species during winemaking [14,80].

Figure 5. Effect of the deliberate addition of Hanseniaspora starter cultures on a diverse set of foodstuffs. References for beer [4,128,129,230–232], cocoa [233–235], coffee [236,237], citrus [238–240], apple [241–245], tequila [118,246,247], other [248–252].

Table 1. Summary of biocontrol trials where Hanseniaspora strains were employed.

Species	Effect	Reference
H. clermontiae	Strong protease activity along with inhibitory action against B. cinerea and P. expansum growth on lab media	[135]
H. guilliermondii	Inhibits mycelial growth of Aspergillus spp.-infected grapes	[136]
H. guilliermondii	Inhibits the growth of the pineapple pathogen F. guttiforme	[253]
H. guilliermondii	Reduces incidence of disease caused by P. digitatum on various citrus fruits	[254]
H. guilliermondii	Displays in vitro antifungal activity against F. graminearum	[255]
H. opuntiae	Releases compounds inhibiting B. cinerea and C. cassiicola growth on Arabidopsis thaliana and Glycine max	[143]
H. opuntiae	Inhibits growth and mycotoxin production of A. flavus attributed to its volatile organic compounds	[151]
H. opuntiae	Inhibits the growth of A. flavus which is enhanced when co-cultured with lactic acid bacteria	[256]
H. opuntiae	Produces volatiles inhibiting mycelial growth of P. italicum and B. cinerea	[257]
H. opuntiae	Reduces radial growth of fig pathogens P. expansum, C. cladosporoides, B. cinerea, and M. laxa on lab media	[258]
H. opuntiae	Displays in vitro antifungal activity against B. cinerea	[259]
H. osmophila	Produces an array of diffusible compounds capable of inhibiting the growth of several fungal pathogens of table grapes	[260]
H. osmophila	Together with G. cerinus inhibits in vitro mycelial growth of Botrytis, Aspergillus, Penicillium, and Rhizopus	[261]
H. uvarum	Exhibits strong inhibitory action against A. carbonarius, B. cinerea, and P. expansum growth on lab media	[135]
H. uvarum	Inhibits growth of P. digitatum and P. italicum attributed to its phenyl ethanol production promoting adhesion	[137]
H. uvarum	Inhibits P. digitatum and P. italicum growth attributed to its 2-phenylethanol production	[138]
H. uvarum	Produces trans-cinnamaldehyde which were shown to inhibit B. cinerea-infection in cherry tomatoes	[139]
H. uvarum	Supplemented with trehalose induces defence-related enzyme activities in table grapes combatting A. tubingensis	[140]
H. uvarum	Produces volatile organics and induce defence‐related enzyme production in strawberries	[141]
H. uvarum	With β-aminobutyric acid shows inhibition against the growth of B. cinerea and A. alternata in kiwifruit	[144]
H. uvarum	Together with phosphatidylcholine inhibits spore germination and mycelial growth of P. digitatum in oranges	[145]
H. uvarum	With the addition of ammonium molybdenum reduces grey mold caused by B. cinerea in grapes	[146]
H. uvarum	With the addition of trehalose reduces grape rot caused by A. tubingensis and P. commune	[147]
H. uvarum	Along with salicylic acid or sodium bicarbonate shows decreased disease incidence of grape berries infected with B. cinerea	[148]
H. uvarum	Certain strains reduce ochratoxin levels within a grape must fermentation	[150]
H. uvarum	Inhibits growth and mycotoxin production of A. flavus attributed to its volatile organic compounds	[151]
H. uvarum	Inhibits the growth of mycotoxigenic fungi and detoxify the major mycotoxins aflatoxin B1 and ochratoxin in grape berries	[152]
H. uvarum	Reduces incidence of disease caused by P. digitatum on various citrus fruits	[254]
H. uvarum	Displays in vitro antifungal activity against B. cinerea	[259]
H. uvarum	Reduces postharvest mold decay in strawberries	[262]
H. uvarum	Reduces natural decay development caused by B. cinerea on grape berries	[263]
H. uvarum	Limits blue mold decay caused by P. italicum on navel orange fruit	[264]
H. uvarum	Produces volatile compounds capable of inhibiting growth of A. ochraceaus in coffee beans	[265]
H. uvarum	Provides effective control against rot caused by Rhizopus of postharvest stone fruits	[266]
H. uvarum	Reduces decay caused by the green and blue molds caused by P. digitatum and P. italicum on a commercial scale	[267]
H. uvarum	Reduces postharvest decay of grapes and peach caused by R. stolonifera and grey mold by B. cinerea	[268]
H. uvarum	Controls brown rot in postharvest plum caused by M. fructicola attributing to chitinase activity and volatile organic compounds	[269]
H. uvarum	Reduces disease caused by P. expansum, M. fructicola and B. cinerea in peaches and nectarines	[270]
H. uvarum	Reduces the incidence of decay, caused by B. cinerea in strawberries	[271]
H. uvarum	Shows inhibition of B. cinerea-infections on grape, apples, and peaches	[272]
H. uvarum	Shows reduction in chili fruit rot caused by C. capsici	[273]
Full species names of fungal pathogens Aspergillus  A. alternata  A. carbonarius  A. flavus  A. ochraceaus  A. tubingensis Botrytis  B. cinerea Cladosporium  C. cladosporoides	Colletotrichum  C. capsici Corynespora  C. cassiicola Fusarium  F. graminearum  F. guttiforme Monilinia  M. fructicola  M. laxa	Penicillium  P. commune  P. digitatum  P. expansum  P. italicum Rhizopus  R. stolonifera

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