Figures & data
Figure 1. Expression of CAF-related genes in breast cancer tissues and normal tissues. Compared with normal breast tissue, THY1, PDPN, FAP, S100A4, DPP4, PDGFRA, PDGFRB, and TNC were higher in breast cancer.
![Figure 1. Expression of CAF-related genes in breast cancer tissues and normal tissues. Compared with normal breast tissue, THY1, PDPN, FAP, S100A4, DPP4, PDGFRA, PDGFRB, and TNC were higher in breast cancer.](/cms/asset/f092a648-b46a-4492-acb9-d9cb3916981a/iann_a_2250987_f0001_c.jpg)
Figure 2. The results of GO and KEGG analysis of CAF-related genes. The bar plot (A) and circos plot (C) showed the top five GO terms; the bar plot (B), bubble plot and circos plot (D) showed the top five KEGG pathway terms BP biological process.
![Figure 2. The results of GO and KEGG analysis of CAF-related genes. The bar plot (A) and circos plot (C) showed the top five GO terms; the bar plot (B), bubble plot and circos plot (D) showed the top five KEGG pathway terms BP biological process.](/cms/asset/5007d0bf-ec59-4e43-b9fe-855b2f0f9204/iann_a_2250987_f0002_c.jpg)
Figure 3. The relationship between PDGFRA and immune infiltrates. The correlation between PDGFRA expression and B cell memory (A), B cells naive (B), DCs resting (C), macrophages M0 (D), macrophages M1(E), macrophages M2 (F), mast cells activated (G), mast cells resting (H), monocytes (I), plasma cells (J), T cells CD4 memory resting (K) infiltration. R < 0, negative correlation, R = 0, no correlation, R > 0, positive correlation.
![Figure 3. The relationship between PDGFRA and immune infiltrates. The correlation between PDGFRA expression and B cell memory (A), B cells naive (B), DCs resting (C), macrophages M0 (D), macrophages M1(E), macrophages M2 (F), mast cells activated (G), mast cells resting (H), monocytes (I), plasma cells (J), T cells CD4 memory resting (K) infiltration. R < 0, negative correlation, R = 0, no correlation, R > 0, positive correlation.](/cms/asset/6e6754e1-7721-4eee-acbd-1cbf90a2e81d/iann_a_2250987_f0003_c.jpg)
Figure 4. The relationship between PDGFRB and immune infiltrates. The correlation between PDGFRB expression and B cell memory (A), B cells naive (B), DCs activated (C), DCs resting (D), eosinophils (E), macrophages M1 (F), mast cells activated (G), mast cells resting (H), T cells CD4 memory activated (I), T cells CD4 memory resting (J), T cells CD8 (K), T cells follicular helper (L) infiltration. R < 0, negative correlation, R = 0, no correlation, R > 0, positive correlation.
![Figure 4. The relationship between PDGFRB and immune infiltrates. The correlation between PDGFRB expression and B cell memory (A), B cells naive (B), DCs activated (C), DCs resting (D), eosinophils (E), macrophages M1 (F), mast cells activated (G), mast cells resting (H), T cells CD4 memory activated (I), T cells CD4 memory resting (J), T cells CD8 (K), T cells follicular helper (L) infiltration. R < 0, negative correlation, R = 0, no correlation, R > 0, positive correlation.](/cms/asset/02c4d245-f477-4724-b257-496feb0ade01/iann_a_2250987_f0004_c.jpg)
Figure 5. The relationship between TNC and immune infiltrates. The correlation between TNC expression and B cells naive (A), DCs activated (B), DCs resting (C), eosinophils (D), macrophages M1 (E), macrophages M2 (F), monocytes (G), T cells CD4 memory resting (H), T cells CD8 (I) infiltration. R < 0, negative correlation, R = 0, no correlation, R > 0, positive correlation.
![Figure 5. The relationship between TNC and immune infiltrates. The correlation between TNC expression and B cells naive (A), DCs activated (B), DCs resting (C), eosinophils (D), macrophages M1 (E), macrophages M2 (F), monocytes (G), T cells CD4 memory resting (H), T cells CD8 (I) infiltration. R < 0, negative correlation, R = 0, no correlation, R > 0, positive correlation.](/cms/asset/7f57f6f1-8e89-45d4-bc06-ee5d0407b61a/iann_a_2250987_f0005_c.jpg)
Figure 6. Kaplan–Meier Plots comparing the OS between the high and low groups of PDGFRA (a), PDGFRB (B), TNC (C) in the TCGA-BRCA cohort. p Values from log-rank tests.
![Figure 6. Kaplan–Meier Plots comparing the OS between the high and low groups of PDGFRA (a), PDGFRB (B), TNC (C) in the TCGA-BRCA cohort. p Values from log-rank tests.](/cms/asset/c8ecce31-63d9-409a-8a8a-e0a52dafa3a8/iann_a_2250987_f0006_c.jpg)
Figure 7. Representative images of immunohistochemistry staining IDC-NOS patients tissues showing the expression of TNC. (A) TNC low expression in the CAF; (B) TNC high expression in the CAF; (C) TNC low expression in the tumor cells; (D) TNC high expression in the tumor cells. Magnification ×100, and ×400; the solid black line indicates the scale bar, 100 μm.
![Figure 7. Representative images of immunohistochemistry staining IDC-NOS patients tissues showing the expression of TNC. (A) TNC low expression in the CAF; (B) TNC high expression in the CAF; (C) TNC low expression in the tumor cells; (D) TNC high expression in the tumor cells. Magnification ×100, and ×400; the solid black line indicates the scale bar, 100 μm.](/cms/asset/5f894ed0-c43d-4156-8e15-291ea4747878/iann_a_2250987_f0007_c.jpg)
Table 1. Correlation between TNC expression and clinicopathological characteristics of IDC-NOS patients(n = 160).
Figure 8. Kaplan–Meier curves of the MFS in IDC-NOS by the TNC expression. MFS survival curve of TNC expression in CAF (A) and tumor cells (C); OS survival curve of TNC expression in CAF (B) and tumor cells (D).MFS: metastasis-free survival; OS: overall survival.
![Figure 8. Kaplan–Meier curves of the MFS in IDC-NOS by the TNC expression. MFS survival curve of TNC expression in CAF (A) and tumor cells (C); OS survival curve of TNC expression in CAF (B) and tumor cells (D).MFS: metastasis-free survival; OS: overall survival.](/cms/asset/3ed919c5-2c41-49f0-940b-202fe129714e/iann_a_2250987_f0008_c.jpg)
Table 2. Relationship between clinicopathological characteristics and disease-free survival in patients with ICD-NOS patients.
Supplemental Material
Download ()Data availability statement
Publicly available datasets were analyzed in this study. Data can be found here: https://portal.gdc.cancer.gov/. All of the authors are responsible for the data.