Antioxidant U74389G Improves Glycerol-Induced Acute Renal Failure without Affecting PPARγ Gene

Figures & data

Figure 1. Effect of U74389G on renal damage and function. Urinary excretion of protein (Figure 1a, left) and Na⁺ (right) or plasma creatinine (Figure 1b, left) and BUN (right) in different groups of control or ARF rats treated with vehicle (Veh) or U74389G (UG: 10 mg/kg/day; orally) for 21 days. Values are mean ± SEM. *p < 0.05 versus control; ^#p < 0.05 versus ARF; n = 6 rats/group. Abbreviations: control = untreated rats; Cont+UG = control rats treated with U74389G; ARF = glycerol-induced acute renal failure; ARF+Veh = ARF rats treated with vehicle; ARF+UG = ARF rats treated with U74389G.

Figure 2. Effect of U74389G on free radical activity and NO production. Plasma 8-Isoprostane (Figure 2a) and urinary nitrite excretion (Figure 2b) in different groups of control or ARF rats treated with vehicle (Veh) or U74389G (UG: 10 mg/kg/day; orally) for 21 days. Values are mean ± SEM. *p < 0.05 versus control; ^#p < 0.05 versus ARF; n = 6 rats/group. Abbreviations: control = untreated rats; Cont+UG = control rats treated with U74389G; ARF = glycerol-induced acute renal failure; ARF+Veh = ARF rats treated with vehicle; ARF+UG = ARF rats treated with U74389G.

Figure 3. Effect of U74389G on renal micro vascular reactivity and plasma. AII vasoconstrictor response to angiotensin II in renal microvessel (Figure 3a) and plasma angiotensin II level (Figure 3b) in different groups of control or ARF rats treated with vehicle (Veh) or U74389G (UG: 10 mg/kg/day; orally) for 21 days. Values are mean ± SEM. *p < 0.05 versus control; ^#p < 0.05 versus ARF; n = 6 rats/group. Abbreviations: control = untreated rats; Cont+UG = control rats treated with U74389G; ARF = glycerol-induced acute renal failure; ARF+Veh = ARF rats treated with vehicle; ARF+UG = ARF rats treated with U74389G).

Figure 4. Effect of U74389G on PPARγ protein expression or activity and mRNA in renal microvessel. PPARγ protein expression (Figure 4a), activity (Figure 4b), and mRNA expression (Figure 4c) in the renal microvascular homogenates from control and ARF rats treated with U74389G (UG: 10 mg/kg/day; orally) or vehicle (Veh) for 21 days. Values are mean ± SEM. *p < 0.05 versus control; n = 6 rats/group. Abbreviations: control = untreated rats; Cont+UG = control rats treated with U74389G; ARF = glycerol-induced acute renal failure; ARF+Veh = ARF rats treated with vehicle; ARF+UG = ARF rats treated with U74389G.

Figure 5. Effect of U74389G on NFκB protein expression. Transcription factor NFκB protein expression in the microvascular homogenate (nuclear fraction) from kidneys of control and ARF rats treated with U74389G (UG: 10 mg/kg/day; orally) or vehicle (Veh) for 21 days. Values are mean ± SEM. *p < 0.05 versus control; ^#p < 0.05 versus ARF; n = 6 rats/ group. Abbreviations: control = untreated rats; Cont+UG = control rats treated with U74389G; ARF = glycerol-induced acute renal failure; ARF+Veh = ARF rats treated with vehicle; ARF+UG = ARF rats treated with U74389G.