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Laboratory Study

Inhibitory effects of taraxasterol and aqueous extract of Taraxacum officinale on calcium oxalate crystallization: in vitro study

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Pages 298-305 | Received 14 Jul 2017, Accepted 06 Mar 2018, Published online: 05 Apr 2018

Figures & data

Figure 1. Effects of potassium citrate (100, 150, 200, and 350 mg/mL) (p < .01) (A), T. officinale extract (1, 2, 4 and 8 mg/mL) (p < .001) (B), and taraxasterol (2.5, 5, 7.5 and 12.5 μg/mL) (p < .05) (C) on absorbance in synthetic urine specimen.

Figure 1. Effects of potassium citrate (100, 150, 200, and 350 mg/mL) (p < .01) (A), T. officinale extract (1, 2, 4 and 8 mg/mL) (p < .001) (B), and taraxasterol (2.5, 5, 7.5 and 12.5 μg/mL) (p < .05) (C) on absorbance in synthetic urine specimen.

Figure 2. Effects of potassium citrate (100, 150, 200 and 350 mg/mL) (p < 0.01) (A), T. officinale extract (1, 2, 4 and 8 mg/mL) (B), and taraxasterol (2.5, 5, 7.5 and 12.5 μg/mL) (C) on %inhibition of nucleation of calcium oxalate crystallization in synthetic urine specimen.

Figure 2. Effects of potassium citrate (100, 150, 200 and 350 mg/mL) (p < 0.01) (A), T. officinale extract (1, 2, 4 and 8 mg/mL) (B), and taraxasterol (2.5, 5, 7.5 and 12.5 μg/mL) (C) on %inhibition of nucleation of calcium oxalate crystallization in synthetic urine specimen.

Figure 3. Effects of taraxasterol (2.5, 5, 7.5 and 12.5 μg/mL), Taraxacum officinale extract (1, 2, 4 and 8 mg/mL) and potassium citrate (100, 150, 200 and 350 mg/mL) on number of CaOx crystals. *p < .05, **p < .01, and ***p < .001 different from control.

Figure 3. Effects of taraxasterol (2.5, 5, 7.5 and 12.5 μg/mL), Taraxacum officinale extract (1, 2, 4 and 8 mg/mL) and potassium citrate (100, 150, 200 and 350 mg/mL) on number of CaOx crystals. *p < .05, **p < .01, and ***p < .001 different from control.

Figure 4. Light microscopic photographs of crystal formation in control group (A), presence of taraxasterol (B), Taraxacum officinale extract (C), and potassium citrate (D) (×400). COD: CaOx dihydrate (bipyramidal-shaped) and COM: CaOx monohydrate (oval-shaped).

Figure 4. Light microscopic photographs of crystal formation in control group (A), presence of taraxasterol (B), Taraxacum officinale extract (C), and potassium citrate (D) (×400). COD: CaOx dihydrate (bipyramidal-shaped) and COM: CaOx monohydrate (oval-shaped).

Table 1. Effects of potassium citrate, aqueous extract of T. officinale and taraxasterol on the number and morphology of CaOx crystals (COM and COD) and the diameter of COD crystals.