Whortleberry protects kidney against the cisplatin-induced nephrotoxicity: an experimental study

Figures & data

Table 1. Experimental study.

Group	Rat numbers
Control	8
Ethanol	8
Whortleberry 100 mg	8
Cisplatin	8
Cisplatin + Whortleberry 100 mg-treated	8
Cisplatin + Whortleberry 200 mg-treated	8

Figure 1. Proximal and distal tubules area (μm2) were calculated using The Olympus DP2-BSW (Ver.2.1 to Ver.2.2, Build 6212, Tokyo, Japan) software system.

Table 2. Biochemical data.

Group	Kidney
	TAS	TOS
Control	9.10 ± 0.28^d^,^e^,^f	27.09 ± 0.90^c^,^d^,^e^,^f
Ethanol	8.57 ± 0.52^c^,^d^,^e	30.03 ± 0.83^a^,^c^,^d^,^e^,^f
Whortleberry 100 mg control	9.82 ± 0,57^b^,^f	23.27 ± 0.70^a^,^b^,^f
Cisplatin	7.86 ± 0.61^a^,^c^,^d^,^e	33.02 ± 0.70^a^,^b^,^c^,^d^,^e
Cisplatin + whortleberry 100 mg	9.91 ± 0.33^a^,^b^,^f	23.78 ± 0.76^a^,^b^,^f
Cisplatin + whortleberry 200 mg	10.43 ± 0.41^a^,^b^,^f	22.46 ± 0.99^a^,^b^,^f

^a p < .05 versus to control group.

^b p < .05 versus to ethanol group.

^c p < .05 versus to whortleberry 100 mg control group.

^d p < .05 versus to cisplatin + whortleberry 100 mg group.

^e p < .05 versus to cisplatin + whortleberry 200 mg group.

^f p < .05 versus to cisplatin group.

Figure 2. Light microscopic photographs from kidney tissue sections stained with H&E.

Figure 3. Light microscope image of testicular tissue by light microscopy. Caspase-3 staining.

Table 3. Proximal tubules area measurement (µm²) data.

Group	Proximal tubules area measurement (Mean ± Standard deviation)
Control	2646.42 ± 292.15^a^,^c
Ethanol	2618.13 ± 421.87^c^,^d
Whortleberry 100 mg	3265.65 ± 887.97^a^,^c
Cisplatin	5839.64 ± 1269.50^b
Cisplatin + whortleberry 100 mg	3541.78 ± 2386.53^a^,^c^,^d
Cisplatin + whortleberry 200 mg	2811.12 ± 483.26^a^,^c^,^e

^a p > .05 versus to control group.

^b p < .05 versus to control group.

^c p < .05 versus to cisplatin group.

^d p > .05 versus to cisplatin + whortleberry 200 mg group.

^e p > .05 versus to cisplatin + whortleberry 100 mg group.

Table 4. Distal tubules area measurement (µm²) data.

Group	Distal tubules area measurement (Mean ± Standard deviation)
Control	3602.18 ± 271.87^a^,^c
Ethanol	3890.18 ± 341.13^c^,^d
Whortleberry 100 mg	3844.42 ± 360.90^a^,^c
Cisplatin	5619.00 ± 693.77^b
Cisplatin + whortleberry 100 mg	3014.32 ± 588.33^a^,^c^,^d
Cisplatin + whortleberry 200 mg	3308.31 ± 582.82^a^,^c^,^e

^a p > .05 versus to control group.

^b p < .05 versus to control group.

^c p < .05 versus to cisplatin group.

^d p > .05 versus to cisplatin + whortleberry 200 mg group.

^e p > .05 versus to cisplatin + whortleberry 100 mg group.

Table 5. Caspase-3 positive numerical density measurement (mm³) data.

Group	Caspase-3 positive numerical density (Mean ± Standard deviation)
Control	4.33 ± 1.03^c^,d
Ethanol	4.67 ± 2.16^a^,c,d
Whortleberry 100 mg	4.83 ± 2.93^a^,c
Cisplatin	44.17 ± 17.53^b
Cisplatin + whortleberry 100 mg	13.00 ± 3.37^a^,c,d
Cisplatin + whortleberry 200 mg	11.25 ± 4.89^a^,c,e

^a p > .05 versus to control group.

^b p < .05 versus to control group.

^c p < .05 versus to cisplatin group.

^d p > .05 versus to cisplatin + whortleberry 200 mg group.

^e p > .05 versus to cisplatin + whortleberry 100 mg group.