Figures & data
Table 1. The primer sequences used for RT-PCR.
Figure 1. Changes in the viability of HK-2 cells were assessed after the HR treatment and BB-94 intervention using CCK-8 kits. All experiments were repeated three times, and the mean values are presented and compared (*p < .05).
![Figure 1. Changes in the viability of HK-2 cells were assessed after the HR treatment and BB-94 intervention using CCK-8 kits. All experiments were repeated three times, and the mean values are presented and compared (*p < .05).](/cms/asset/96713389-b216-4847-a6f9-6843c2646925/irnf_a_1930558_f0001_b.jpg)
Figure 2. Relative fluorescence intensity and ratio of ROS + HK-2 cells. (A) Fluorescence images of HK-2 cells captured using a fluorescence microscope. (B) Quantitative analysis of the relative fluorescence intensity. (C) Flow cytometry plots of HK-2 cells. (D) Quantitative flow cytometry data. All experiments were repeated three times, and the mean values are presented and compared (**p < .01 and ***p < .005).
![Figure 2. Relative fluorescence intensity and ratio of ROS + HK-2 cells. (A) Fluorescence images of HK-2 cells captured using a fluorescence microscope. (B) Quantitative analysis of the relative fluorescence intensity. (C) Flow cytometry plots of HK-2 cells. (D) Quantitative flow cytometry data. All experiments were repeated three times, and the mean values are presented and compared (**p < .01 and ***p < .005).](/cms/asset/2b0c410f-53ad-4d83-9a6a-50bb17383139/irnf_a_1930558_f0002_c.jpg)
Figure 3. Levels of the MMP-2, MMP-9, and TIMP-1 mRNAs in HK-2 cells. (A) Real-time PCR analysis of levels of the MMP-2 mRNA in HK-2 cells. (B) Levels of the MMP-9 mRNA in HK-2 cells. (C) Levels of the TIMP-1 mRNA in HK-2 cells. All experiments were repeated three times, and the mean values are presented and compared (*p < .05; **p < .005; and ****p < .001).
![Figure 3. Levels of the MMP-2, MMP-9, and TIMP-1 mRNAs in HK-2 cells. (A) Real-time PCR analysis of levels of the MMP-2 mRNA in HK-2 cells. (B) Levels of the MMP-9 mRNA in HK-2 cells. (C) Levels of the TIMP-1 mRNA in HK-2 cells. All experiments were repeated three times, and the mean values are presented and compared (*p < .05; **p < .005; and ****p < .001).](/cms/asset/c4f799b8-2dc3-4cb9-a1de-113f2e9484ae/irnf_a_1930558_f0003_b.jpg)
Figure 4. Levels of the MMP-9 and MMP-2 proteins in HK-2 cells. (A) Western blot analysis of MMP-2 protein levels. (B) Quantitation of the Western blot analysis of MMP-2 protein levels. (C) Western blot analysis of MMP-9 protein levels. (D) Quantitation of the Western blot analysis of MMP-9 protein levels. All experiments were repeated three times, and the mean values are presented and compared (*p < .05).
![Figure 4. Levels of the MMP-9 and MMP-2 proteins in HK-2 cells. (A) Western blot analysis of MMP-2 protein levels. (B) Quantitation of the Western blot analysis of MMP-2 protein levels. (C) Western blot analysis of MMP-9 protein levels. (D) Quantitation of the Western blot analysis of MMP-9 protein levels. All experiments were repeated three times, and the mean values are presented and compared (*p < .05).](/cms/asset/5b664374-1c99-48d9-8f03-2f6cb5c45032/irnf_a_1930558_f0004_b.jpg)
Figure 5. Levels of the CD-11b, CD-18, and MMP-9 mRNAs in PMNs. (A) Real-time PCR analysis of levels of the CD-11b mRNA in PMNs. (B) Levels of the CD-18 mRNA in PMNs. (C) Levels of the MMP-9 mRNA in PMNs. All experiments were repeated three times, and the mean values are presented and compared (*p < .05 and **p < .01).
![Figure 5. Levels of the CD-11b, CD-18, and MMP-9 mRNAs in PMNs. (A) Real-time PCR analysis of levels of the CD-11b mRNA in PMNs. (B) Levels of the CD-18 mRNA in PMNs. (C) Levels of the MMP-9 mRNA in PMNs. All experiments were repeated three times, and the mean values are presented and compared (*p < .05 and **p < .01).](/cms/asset/82629c79-369a-42fa-bffc-ac90b4f6a121/irnf_a_1930558_f0005_b.jpg)
Supplemental Material
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Download PDF (132.8 KB)Data availability statement
The data used to support the findings of this study are included in the article.