ABSTRACT
Lactic acid bacteria (LAB) have gained much attention recently due to their probiotic attributes with anti-ACE and anti-glycation potentials targeting management of diabetes. This study was designed to isolate LAB from human milk and to evaluate their potential for inhibitory activities on ACE and AGEs formation using in vitro assay models. Among 12 LAB isolates, many strains exhibited strong probiotic attributes including tolerance to low pH, high salt, gastric, and intestinal juices with higher cell adhesion. Further, cell-free extracts of LAB tested for in vitro anti-ACE and anti-AGEs activities evidenced Lacticaseibacillus casei MKU1, Lacticaseibacillus paracasei MKU2, Lactiplantibacillus pentosus MKU3, L. paracasei MKU7, L. delbrueckii GERU3 and Lactobacillus delbrueckii MKU10 as strong ACE and AGEs inhibitors. Subsequently, Principal Component Analysis showed a positive correlation with anti-ACE (p ≤ .01) and anti-glycation (p ≤ .05) activity of LAB. Overall, this study suggests that six Lactobacillus isolates of human milk with superior probiotic attributes looks promising as potential probiotic strains.
Acknowledgments
Authors gratefully acknowledge the Indian Council of Medical Research (ICMR), New Delhi, India [No. 5/4/5-4/Diab.-16-NCD-II] for their financial support through a research grant to BA. We also thank DST-PURSE, DST-FIST, UGC-SAP and MKU-RUSA Phase II programs of Madurai Kamaraj University for the infrastructure and other facilities.
Ethical clearance
This study concerning milk sample donors were reviewed and approved by the Institutional Ethical Committee of Madurai Kamaraj University, Madurai, INDIA.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Supplementary material
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