A comparative study on adhesion and recovery of potential probiotic strains of Lactobacillus spp. by in vitro assay and analysis of human colon biopsies

Figures & data

Table I.  Adhesion of Lactobacillus strains used in this study to IPEC-J2 cells.

Bacterial strains	Adhesion ( SD),%
Preparation A
L. rhamnosus 19070	60±1
L. reuteri 12246	67±3
L.casei F19	6±2
Preparation B
L. casei D12	7±2
L. casei Q85	8±3
L. casei Z11	26±6
L. plantarum Q47	13±5

Adhesion is expressed as the percentage of bacterial counts adhered to IPEC-J2 cells to the total number of bacteria added. The means of two independent determinations±standard deviation (SD) are presented.

Table II.  Recovery of ingested bacterial preparations A and B from the colon biopsies and faecal samples collected in the human intervention studies.

		Number of colonies from colon biopsies and faeces (strain number/total number)*
Preparation/subjects	Bacterial strains	Ascending colon	Transverse colon	Descending colon	Faeces
Preparation A
Subject A1	L. rhamnosus 19070	21/25 (84%)	22/25 (88%)	24/25 (96%)	6/50 (12%)
	L. reuteri 12246	1/25 (4%)	0/25 (0%)	0/25 (0%)	34/50 (68%)
	L.casei F19	3/25 (12%)	3/25 (12%)	1/25 (4%)	1/50 (2%)
Subject A2	L. rhamnosus 19070	1/25 (4%)	2/25 (8%)	0/25 (0%)	NA
Subject A3	L. rhamnosus 19070	2/4 (50%)	11/17 (64%)	2/6 (40%)	NA
	L.casei F19	2/4 (50%)	6/17 (36%)	3/6 (50%)	NA
Subject A4	Not identified	NG	NG	NG	0/25 (0%)
Preparation B
Subject B1	L. casei Q85	9/10 (90%)	5/6 (83%)	NG	4/56 (8%)
	L. casei Z11	1/10 (10%)	0/6 (0%)	NG	7/56 (13%)
Subject B2	L. casei Q85	2/7 (30%)	4/24 (18%)	4/17 (24%)	0/48 (0%)
	L. casei Z11	1/7 (15%)	8/24 (30%)	0/17 (0%)	1/48 (2%)
Subject B3	L. casei Z11	0/3 (0%)	1/24 (4%)	1/12 (8%)	NA
Subject B4	Not identified	0/19 (0%)	0/16 (0%)	0/21 (0%)	0/25 (0%)

NA, samples were not analysed; NG, no growth of colonies on MRS agar.

*Number of identified colonies to the total number of colonies isolated from colon biopsies and faeces and analysed by Rep-PCR.