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Brief Report

A whole blood-based functional assay to characterize immunoglobulin A effector functions

, , , &
Article: 2341629 | Received 19 Sep 2023, Accepted 06 Apr 2024, Published online: 14 Apr 2024

Figures & data

Figure 1. IgA subclasses show different activation profiles upon whole blood incubation. (a) Principal component analysis after selection of most discriminative parameters identified in Figure S3. (b) Box plots of the best discriminants for granulocytes (b), monocytes (c) and NK cells (d). Each shape represents a different donor (n = 10). Triplicate MFI average are represented excluding the conditions in which we obtained CVs > 30%. Triplicates with CVs between 20 and 30% are represented in yellow. Statistical significance was determined by a nonparametric Wilcoxon test with multiple comparisons, with *p < 0.05.

Figure 1. IgA subclasses show different activation profiles upon whole blood incubation. (a) Principal component analysis after selection of most discriminative parameters identified in Figure S3. (b) Box plots of the best discriminants for granulocytes (b), monocytes (c) and NK cells (d). Each shape represents a different donor (n = 10). Triplicate MFI average are represented excluding the conditions in which we obtained CVs > 30%. Triplicates with CVs between 20 and 30% are represented in yellow. Statistical significance was determined by a nonparametric Wilcoxon test with multiple comparisons, with *p < 0.05.
Supplemental material

Supplemental Material

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Data availability statement

Raw data can be shared upon reasonable request.