Modulatory effect of hydrogen peroxide on tumoral lymphocytes proliferation

Abstract

BW 5147 (murine lymphoma cell line). We analyzed the effect of H₂O₂ in cell proliferation testing nitric oxide and apoptosis. Enzymes involved in the regulation of H₂O₂ levels as superoxide dismutase (SOD) and peroxidase (PER) were analyzed. H₂O₂ exerted a biphasic effect. The inhibitory effect of H₂O₂ was related to the activation of the ERK and P38 pathway, NO production and apoptosis. The high proliferation was associated with a low level of H₂O₂ related to a low SOD and a high PER activities. Drugs capable of producing an increase in H₂O₂ levels could be used in cancer.

Keywords::

• Hydrogen peroxide
• lymphoma cell line
• ERK pathway
• P-38 pathway
• nitric oxide

Acknowledgment

This work was supported by funds provided by PIP CONICET 5232 and Project 9601 UNSL. Roberto Davicino is a postdoctoral fellow of CONICET.