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Biofouling
The Journal of Bioadhesion and Biofilm Research
Volume 38, 2022 - Issue 5
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Research Article

High-throughput method development for in-situ quantification of aquatic phototrophic biofilms

ORCID Icon, & ORCID Icon
Pages 521-535 | Received 02 Feb 2022, Accepted 21 Jun 2022, Published online: 06 Jul 2022

Figures & data

Figure 1. In vitro autofluorescence measurements of 9-day Cylindrotheca closterium biofilms, as recorded with (A) light (top) and fluorescence microscopy (bottom) images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where bars represent standard error.

Figure 1. In vitro autofluorescence measurements of 9-day Cylindrotheca closterium biofilms, as recorded with (A) light (top) and fluorescence microscopy (bottom) images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where bars represent standard error.

Figure 2. In vitro autofluorescence measurements of 9-day Nitzschia thermaloides biofilms, as recorded with (A) light (top) and fluorescence (bottom) microscopy images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where error bars represent standard error.

Figure 2. In vitro autofluorescence measurements of 9-day Nitzschia thermaloides biofilms, as recorded with (A) light (top) and fluorescence (bottom) microscopy images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where error bars represent standard error.

Figure 3. In situ autofluorescence measurements of 19-day natural phototrophic biofilms in Southsea Marina UK during Spring 2017, as recorded with (A) light (top) and fluorescence microscopy (bottom) images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where error bars represent standard error.

Figure 3. In situ autofluorescence measurements of 19-day natural phototrophic biofilms in Southsea Marina UK during Spring 2017, as recorded with (A) light (top) and fluorescence microscopy (bottom) images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where error bars represent standard error.

Figure 4. In situ autofluorescence measurements of 28-day natural phototrophic biofilms in Southsea Marina UK during Summer 2017, as recorded with (A) light (top) and fluorescence microscopy (bottom) images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where error bars represent standard error.

Figure 4. In situ autofluorescence measurements of 28-day natural phototrophic biofilms in Southsea Marina UK during Summer 2017, as recorded with (A) light (top) and fluorescence microscopy (bottom) images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where error bars represent standard error.

Figure 5. In situ autofluorescence measurements of 30-day natural phototrophic biofilms in Southsea Marina UK during Autumn 2017, as recorded with (A) light (top) and fluorescence microscopy (bottom) images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where error bars represent standard error.

Figure 5. In situ autofluorescence measurements of 30-day natural phototrophic biofilms in Southsea Marina UK during Autumn 2017, as recorded with (A) light (top) and fluorescence microscopy (bottom) images (scale bars = 20 μm), and (B) merged fluorescence microscopy and plate reader fluorescence records, where error bars represent standard error.

Figure 6. Scatterplot of general linear model for fluorescence measurements between plate reader and microscopy, testing (a) in vitro monoculture biofilms (R2 = 0.96), (b) in situ marine phototrophic biofilms (R2 = 0.77), and (c) both in vitro and in situ biofilms (R2 = 0.70).

Figure 6. Scatterplot of general linear model for fluorescence measurements between plate reader and microscopy, testing (a) in vitro monoculture biofilms (R2 = 0.96), (b) in situ marine phototrophic biofilms (R2 = 0.77), and (c) both in vitro and in situ biofilms (R2 = 0.70).
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