Entrapment of Ovalbumin into Liposomes—Factors Affecting Entrapment Efficiency, Liposome Size, and Zeta Potential

Abstract

Various amounts of Ovalbumin (OVA) were encapsulated into positively and negatively charged multilamellar liposomes, with the aim to investigate the entrapment efficiency in different buffers and to study their effects on the liposome size and zeta potential. Results showed that the entrapment efficiency of OVA in anionic liposomes was the same in 10 mM Phosphate Buffer (PB) as in Phosphate-Buffered Saline (PBS; PB + 0.15 M NaCl). Also, liposome size was approximately 1200 nm for all anionic liposomes incorporating OVA. The entrapment efficiency of OVA in cationic liposomes was highly dependent on ionic strength. The size of cationic liposomes was approximately 1200 nm in PBS, regardless of protein content, but increased with the amount of the incorporated protein in PB. Aggregation of cationic liposomes in PB was observed when the mass of the protein was 2.5 mg or greater. The zeta potential of anionic liposomes was negative and of cationic liposomes positive in the whole range of protein mass tested. These results show how different compositions of lipid and aqueous phases can be used to vary the entrapment efficiency, liposome size, and zeta potential—the factors that are of great importance for the use of liposomes as drug carriers.

Keywords:

• entrapment efficiency
• cationic liposomes
• liposome size
• zeta potential
• ovalbumin
• drug delivery

Acknowledgments

The authors thank Dr. Beata Halassy for helpful discussions. The financial support by the Croatian Ministry of Science, Education, and Sports through the projects No. 021-0212432-2431, 021-0212432-2033, 098-0982915-2949 is also gratefully acknowledged.