Specificity and selectivity of arbuscular mycorrhizal fungal polymerase chain reaction primers in soil samples by clone library analyses

Figures & data

Table 1. Primer sets for 18S rDNA of AMF.

Primer set	PCR product length (bp)	References
SSUmAf/LSUmAr + SSUmCf/LSUmBr	1500	Kruger et al. (2009)
GeoA2/Geo11	1800	Schwarzott and Schüßler (2001)
NS31/AM1	550	Simon et al. (1992); Helgason et al. (1998)
AML1/AML2	800	Lee et al. (2008)
AMV4.5NF/AMDGR	300	Sato et al. (2005)

Figure 1. Positions of the primers on rDNA of AMF.

Figure 2. The proportion of AMF sequences obtained from primer set 1, 2, 3 and 4 (P1, P2, P3 and P4) in three soil samples.

Figure 3. The OTUs and Shannon's index (H) of the Glomeromycota in three soil samples using the four primer sets (P1, P2, P3 and P4).

Figure 4. Proportion of 18S rDNA sequences in each cluster using primer sets 2, 3 and 4.

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