Isolation of three genera of microorganisms in lahar-laden soils of Sta. Rita, Pampanga, Philippines through the 16s rRNA gene sequence analysis

Figures & data

Table 1. Soil physical-chemical properties of the three (3) locations in Sta Rita, Pampanga, after being laden with lahar.

LOCATION	PARAMETERS						SOIL TEXTURE (%)			TEXTURAL CLASS
Sta Rita, Pampanga	pH	N (%)	P (ppm)	K (cmol/kg)	CEC (cmol/kg)	E.C. (mS/cm)	Sand	Silt	Clay
1. San Jose	5.32	0.08	115.95	0.35	5.76	0.14	54.79	24.54	20.67	Sandy loam
2. San Isidro	5.4	0.07	23.61	0.27	4.97	0.45	44.75	36.54	18.7	Loam
3. San Juan	4.95	0.15	8.83	0.24	7.77	0.47	33.01	48.35	18.64	Loam

Figure 1. (A) The pink colour in nodules indicates leghemoglobin, (B) sterilisation of nodules in microtubes, (C) Growth of isolates in Yeast Extract Mannitol Agar (YMA) plate.

Figure 2. (A) Root nodules of mung bean under lahar-laden soil, (B) Rhizobia grew on YMA+ Congo red, (C) Rhizobia grew on YMA + Bromothymol Blue (BTB).

Table 2. Characterisation of isolates based on their growth morphology on Yeast Extract Mannitol Agar (YMA) plate medium, including a number of pure isolates with an average number of nodules indicated in parenthesis, isolated from lahar-laden soil of Sta Rita, Pampanga.

REPRESENTATIVE ISOLATES		NO OF PURE ISOLATES	COLONY MORPHOLOGY	FORMATION OF ACID /ALKALINE SUBSTANCES
San Jose	S.Jo	4 (34)	Translucent, offwhite, circular, liquid, mucoid, slightly convex, elevation with entire margin	Alkaline
San Isidro	S.Is	2 (8)	Translucent, pink, irregular, liquid, smooth, slightly convex, elevation with entire margin	Acid
	S.I	1 (8)	Translucent, cream, rod-shaped, liquid, smooth, convex, elevation with entire margin	Acid
San Juan	S.Ju	3 (32)	Opaque, white cream smooth, viscid circular, convex elevation with entire margin	Alkaline
Total No. of pure Isolates and nodules		10 (82)

Figure 3. The phylogenetic analysis of mung bean isolates using 16S rRNA gene sequence from San Jose under lahar sediments.

Figure 4. Dendrogram of genetic diversity of mung bean isolates from San Isidro based on 16S rRNA gene with accession numbers.

Figure 5. The dendrogram shows the genetic diversity of isolates with accession numbers based on 16S rRNA sequence analysis.

Figure 6. The dendrogram shows the phylogenetic diversity of bacteria on 16S rRNA gene sequencing with accession numbers. Isolates were collected in root nodules of mung beans under the lahar-laden soil of San Juan (S.Ju).

Data availability

The data supporting this study's findings are available upon reasonable request.