Advanced search
Publication Cover
Bioscience, Biotechnology, and Biochemistry Volume 79, 2015 - Issue 6
Journal homepage
529
Views
6
CrossRef citations to date
0
Altmetric
Biochemistry & Molecular Biology (Note)

Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase

Toshinori YokooDivision of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, JapanView further author information
,
Ken'ichiro MatsumotoDivision of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, JapanCorrespondence[email protected]
View further author information
,
Takashi OobaDivision of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, JapanView further author information
,
Kenjiro MorimotoDivision of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, JapanView further author information
&
Seiichi TaguchiDivision of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, JapanView further author information
Pages 986-988 | Received 29 Aug 2014, Accepted 11 Dec 2014, Published online: 03 Feb 2015

Figures & data

Fig. 1. The plasmids used in this study.

Notes: phaC: PHA synthase gene, phaB: acetoacetyl-CoA reductase gene (wild type and TS mutant), 35S: 35S cauliflower mosaic virus promoter, NOS: nopaline synthase terminator, Kmr: kanamycin resistance gene, T-LB and T-RB: left and right borders of T-DNA, respectively.

Fig. 1. The plasmids used in this study.Notes: phaC: PHA synthase gene, phaB: acetoacetyl-CoA reductase gene (wild type and TS mutant), 35S: 35S cauliflower mosaic virus promoter, NOS: nopaline synthase terminator, Kmr: kanamycin resistance gene, T-LB and T-RB: left and right borders of T-DNA, respectively.

Fig. 2. RT-PCR of transgenic tobacco harboring phaB and phaC genes.

Notes: 1-3: transformants of pRIphaC-phaB(WT), 4-6: transformants of pRIphaC-phaB(TS), 7: wild-type BY2, and 8: positive control (plasmid).

Fig. 2. RT-PCR of transgenic tobacco harboring phaB and phaC genes.Notes: 1-3: transformants of pRIphaC-phaB(WT), 4-6: transformants of pRIphaC-phaB(TS), 7: wild-type BY2, and 8: positive control (plasmid).

Fig. 3. P(3HB) content in transgenic tobacco BY-2 cells.

Notes: WT: transformants of pRIphaC-phaB(WT), TS: transformants of pRIphaC-phaB(TS).

Fig. 3. P(3HB) content in transgenic tobacco BY-2 cells.Notes: WT: transformants of pRIphaC-phaB(WT), TS: transformants of pRIphaC-phaB(TS).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.