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Organic Chemistry (Note)

Stage-specific quercetin sulfation in the gut of Mythimna separata larvae (Lepidoptera: Noctuidae)

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Pages 38-40 | Received 09 Oct 2013, Accepted 19 Oct 2013, Published online: 16 Apr 2014

Figures & data

Fig. 1. Structures of quercetin (A) and quercetin 4′-O-sulfate (B), and a typical chromatogram of quercetin metabolites in the frass of 3rd and 6th instar M. separata larvae (C).

Note: LC/MS selected ion chromatograms ([M–H]) of quercetin 3-O-glucoside (m/z 463), quercetin (m/z 301), quercetin 4′-O-sulfate (m/z 381), and the total ion chromatogram (TIC) are shown.

Fig. 1. Structures of quercetin (A) and quercetin 4′-O-sulfate (B), and a typical chromatogram of quercetin metabolites in the frass of 3rd and 6th instar M. separata larvae (C).Note: LC/MS selected ion chromatograms ([M–H]−) of quercetin 3-O-glucoside (m/z 463), quercetin (m/z 301), quercetin 4′-O-sulfate (m/z 381), and the total ion chromatogram (TIC) are shown.

Fig. 2. Amounts of quercetin metabolites identified in the frass of 3rd (left) and 6th (right) instar larvae fed the 1% quercetin diet (mean ± SD, n = 5).

Note: Quercetin 3-O-glucoside (Q3G), quercetin (Q), and quercetin 4′-O-sulfate (Q4′S) are shown. Different letters represent significant differences (p < 0.05, Tukey–Kramer HSD test for multiple comparisons).

Fig. 2. Amounts of quercetin metabolites identified in the frass of 3rd (left) and 6th (right) instar larvae fed the 1% quercetin diet (mean ± SD, n = 5).Note: Quercetin 3-O-glucoside (Q3G), quercetin (Q), and quercetin 4′-O-sulfate (Q4′S) are shown. Different letters represent significant differences (p < 0.05, Tukey–Kramer HSD test for multiple comparisons).

Fig. 3. Amounts of quercetin 4′-O-sulfate produced by in vitro incubation with the substrates and homogenates of each part of 6th instar larvae (mean ± SD, n = 5).

Note: Different letters represent significant differences (p < 0.05, Tukey–Kramer HSD test for multiple comparisons).

Fig. 3. Amounts of quercetin 4′-O-sulfate produced by in vitro incubation with the substrates and homogenates of each part of 6th instar larvae (mean ± SD, n = 5).Note: Different letters represent significant differences (p < 0.05, Tukey–Kramer HSD test for multiple comparisons).

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