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Bioscience, Biotechnology, and Biochemistry Volume 78, 2014 - Issue 7
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Microbiology & Fermentation Technology (Notes)

Bactericidal activity of nukacin ISK-1: an alternative mode of action

Urmi RoyLaboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
,
Mohammad Riazul IslamLaboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
,
Jun-ichi NagaoSection of Infection Biology, Department of Functional Bioscience, Fukuoka Dental College, Fukuoka, Japan
,
Hiroshi IidaLaboratory of Zoology, Division of Agricultural Bioresource Sciences, Department of Bioresource Sciences, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
,
Abdullah-Al MahinLaboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
,
Mengqi LiLaboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
,
Takeshi ZendoLaboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
,
Jiro NakayamaLaboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan
&
Kenji SonomotoLaboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka, Japan;Laboratory of Functional Food Design, Department of Functional Metabolic Design, Bio-Architecture Center, Kyushu University, Fukuoka, JapanCorrespondence[email protected]
 show all
Pages 1270-1273 | Received 31 Dec 2013, Accepted 18 Mar 2014, Published online: 18 Jun 2014

Figures & data

Table 1. Susceptibility of different indicator strains to nukacin ISK-1 and nisin A.

Fig. 1. Antibacterial activity of nukacin ISK-1.

CFU values (A) immediately after peptide addition (white bars) and 24 h after peptide addition (black bars) in S. simulans and M. luteus are shown. Membrane depolarization (B), ATP release (C), and K+ leakage (nisin is considered 100% release) (D) were measured on S. simulans, M. luteus, and L. sakei cells with no peptide (gray bars), nukacin ISK-1 (white bars), and nisin (black bars) treatment. About 5X MIC of peptides were used for all experiments. The values shown are the means ± standard errors (error bars) for the three independent experiments.

Fig. 1. Antibacterial activity of nukacin ISK-1.CFU values (A) immediately after peptide addition (white bars) and 24 h after peptide addition (black bars) in S. simulans and M. luteus are shown. Membrane depolarization (B), ATP release (C), and K+ leakage (nisin is considered 100% release) (D) were measured on S. simulans, M. luteus, and L. sakei cells with no peptide (gray bars), nukacin ISK-1 (white bars), and nisin (black bars) treatment. About 5X MIC of peptides were used for all experiments. The values shown are the means ± standard errors (error bars) for the three independent experiments.

Fig. 2. Transmission electron micrographs of M. luteus cells.

Micrographs of the cells treated without peptide (A), with 10X MIC of nisin (B), and nukacin ISK-1 (C) for 2 h. Enlarged cross-sections of the cells without peptide (A’), with nisin (B’), and nukacin ISK-1 (C’). Bars in panels A and A’: 500 nm.

Fig. 2. Transmission electron micrographs of M. luteus cells.Micrographs of the cells treated without peptide (A), with 10X MIC of nisin (B), and nukacin ISK-1 (C) for 2 h. Enlarged cross-sections of the cells without peptide (A’), with nisin (B’), and nukacin ISK-1 (C’). Bars in panels A and A’: 500 nm.

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Related Research Data

The lantibiotic nukacin ISK-1 exists in an equilibrium between active and inactive lipid-II binding states.
Source: Springer Science and Business Media LLC

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