Optimization of culture conditions of Fusarium solani for the production of neoN-methylsansalvamide

Figures & data

Fig. 1. Full mass spectrum of neoN-methylsansalvamide.

Fig. 2. Production of neoN-methylsansalvamide by F. solani KCCM90040 on six cereal grains.

Fig. 3. Effect of environmental conditions on the production of neoN-methylsansalvamide. (a) Temperature. (b) Initial moisture content. (c) Growth time.

Table 1. CCD arrangement and response.

No.	Temperature (°C)	Initial moisture content (%)	Growth time (days)	NeoNMeSSV (mg/kg)
1	15	30	20	150
2	35	40	15	262
3	35	30	20	230
4	35	30	10	248
5	25	40	20	522
6	15	50	10	150
7	25	40	15	652
8	25	40	15	702
9	25	50	15	580
10	25	40	15	628
11	25	40	15	622
12	25	40	15	602
13	35	50	10	270
14	15	30	10	130
15	25	40	15	624
16	25	30	15	508
17	35	50	20	380
18	25	40	10	300
19	15	40	15	150
20	15	50	20	190

Note: neoNMeSSV: neoN-methylsansalvamide.

Table 2. Regression coefficients of the quadratic polynomials as functions of the conditions for the amount of neoN-methylsansalvamide.

Variables	Coefficients	p-values
Intercept	−2755.79
Temp.	1333.17570	<0.0001
Initial moisture content	54.08784	0.0081
Growth time	35.69614	<0.0001
Temp. × Temp.	−2.66136	<0.0001
Initial moisture content × Initial moisture content	−0.78136	0.0018
Growth time × Growth time	−2.44545	0.0082
Temp. × Initial moisture content	−0.03125	0.7803
Temp. × Growth time	0.68750	0.0103
Initial moisture content × Growth time	0.82750	0.0035

Table 3. ANOVA table.

	Sum of squares	Degrees of freedom	MS	p-values
Model	811848.1	9	90205.34	<0.0001
Residual	9518.461	10	951.8461
Lack of fit	3379.128	5	675.8256	0.7359
Pure error	6136.333	5	1227.867
R^2	0.988411
Adjusted R^2	0.977982

Fig. 4. Response surface plots for the production of neoN-methylsansalvamide. (a) Temperature × Initial moisture content. (b) Temperature × Growth time. (c) Initial moisture content × Growth time.