Fimbriae and lipopolysaccharides are necessary for co-aggregation between Lactobacilli and Escherichia coli

Figures & data

Table 1. Co-aggregation between E. coli MG1655 and various LAB.

Bacteria	Co-aggregation
L. casei subsp. rhamnosus NBRC3831	+
L. delbrueckii NBRC3202	+
L. delbrueckii subsp. bulgaricus NBRC13953	+
L. acidophilus NBRC13951	+
L. johnsonii NBRC13952	+
L. plantarum NBRC15891	+
L. plantarum NCIMB8826	+
Leuconostoc fructosum NBRC3516	+
Lactococcus lactis subsp. lactis NBRC12007	+
L. plantarum ML11–11^*	+

* Isolate showing high co-aggregation with yeast.²⁴⁾.

Fig. 1 Co-aggregation between E. coli MG1655 and L. casei NBRC3831 on a Polystyrene Dish.

Note: Unstained co-aggregates (A) and Gram-stained co-aggregates (B) are shown. Scale bar, 10 μm.

Table 2. The co-aggregation ability of E. coli mutants lacking various outer membrane structures.

E. coli strains	Description	Co-aggregation
MG1655	Wild type	++
BW25113	Parent strain of the mutants	++
JW1908	ΔfliC	++
JW1025	ΔcsgA	++
JW4277	ΔfimA	–
JW3596	ΔrfaC	–

Katakura Y, Sano R, Hashimoto T, Ninomiya K, Shioya S. Lactic acid bacteria display on the cell surface cytosolic proteins that recognize yeast mannan. Appl. Microbiol. Biotechnol. 2010;86:319–326.

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