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Food & Nutrition Science

Biotin-deficient diet induces chromosome misalignment and spindle defects in mouse oocytes

, &
Pages 292-299 | Received 25 Jul 2014, Accepted 12 Sep 2014, Published online: 10 Oct 2014

Figures & data

Table 1. Diet compositions.

Table 2. Effects of biotin deficiency.

Table 3. Effects of biotin restoration.

Fig. 1. Spindle abnormalities in oocytes induced by biotin deficiency.

Notes: Representative examples of meiotic spindles in oocytes from indicated mice (n  = 37–38 oocytes analyzed per group) after labeling α-tubulin antibody (green) and counter-staining DNA with DAPI (aqua blue). (A) control group, (B) biotin-deficient group.

Fig. 1. Spindle abnormalities in oocytes induced by biotin deficiency.Notes: Representative examples of meiotic spindles in oocytes from indicated mice (n  = 37–38 oocytes analyzed per group) after labeling α-tubulin antibody (green) and counter-staining DNA with DAPI (aqua blue). (A) control group, (B) biotin-deficient group.

Fig. 2. Higher frequency of abnormal oocytes induced by biotin deficiency not restored by re-feeding of biotin-containing diet.

Notes: Abnormal oocyte number is the summation of chromosome alignment and spindle defect in oocytes. (A) Day 22, (B) Day 42. (C) control; BD; biotin deficient group; R, recovery group. n = 37–54 oocytes analyzed per group.

Fig. 2. Higher frequency of abnormal oocytes induced by biotin deficiency not restored by re-feeding of biotin-containing diet.Notes: Abnormal oocyte number is the summation of chromosome alignment and spindle defect in oocytes. (A) Day 22, (B) Day 42. (C) control; BD; biotin deficient group; R, recovery group. n = 37–54 oocytes analyzed per group.

Fig. 3. Meiosis aneuploidy induced by biotin deficiency, not restored by biotin re-feeding.

Notes: Representative examples of meiotic spindles in oocytes from indicated mice (n = 41–66 oocytes analyzed per group) after labeling α-tubulin antibody (green) and counter-staining DNA with DAPI (aqua blue). (A) control group, (B) biotin-deficient group, and, (C) recovery group.

Fig. 3. Meiosis aneuploidy induced by biotin deficiency, not restored by biotin re-feeding.Notes: Representative examples of meiotic spindles in oocytes from indicated mice (n = 41–66 oocytes analyzed per group) after labeling α-tubulin antibody (green) and counter-staining DNA with DAPI (aqua blue). (A) control group, (B) biotin-deficient group, and, (C) recovery group.

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