Figures & data
Table 1. The genes affected by excess of extracellular cystine in E. coli.
Fig. 1. Induction of yijE promoter and repression of yhdT and yihN promoters by addition of cystine.
Notes: The reporter strains containing yijE-lacZ (a), yhdT-lacZ (b), and yihN-lacZ (c) (see Materials and methods) were grown in M9-glucose with (black bars) and without (white bars) cystine (10 μM) until log phase, and then subjected to β-galactosidase assay.
Fig. 2. Resistance to cystine of E. coli by expression of yijE gene.
Notes: BW25113 harboring pQE80L (a) and pYijE (b) were grown in M9-glucose with (triangle) and without (circle) cystine (80 μM). The growth of cultures with reciprocal shaking of an elbow glass tube was automatically measured by TVS062CA (Advantec). This measurement of growth curve was performed three times for each experiment with independent cultures. The fluctuation level among three determinations was less than 20%.
Fig. 3. Sensibility to cystine of the yijE-deficient mutant.
Notes: BW25113 (a) and JW4303 (b) were grown in M9-glucose with (triangle) and without (circle) cystine (20 μM). The growth of cultures with reciprocal shaking of a glass tube was measured by its turbidity at OD 600 nm. This measurement of growth curve was performed three times for each experiment with independent cultures. The fluctuation level among three determinations was less than 15%. The p-values from Student’s t-distribution were 0.29, 0.33, 021, 0.04, <0.01, and 0.39 at 0, 1, 2, 4, 6, and 24 h, respectively, shown in (b).
