Figures & data
Fig. 1. The novel hydrostatic pressure bioreactor.
(a) Double package of monolayer sample filled up with culture medium and RO water, (b) schematic diagram of the bioreactor; Shimadzu Microservo device was utilized for compression loading onto the double-packed sample, (c) pressure measured during loading by the pressure sensor.
Fig. 2. mRNA Expression of chondrogenic markers in ATDC5 cells under hydrostatic pressure measured by real-time PCR.
(a) Sox9 (b) Acan (c) Col2a1. Graphs show the fold change of chondrogenic markers mRNA expression relative to RPL13a mRNA normalized to the control mean. The bars represent the mean ± standard error. All the mRNA expressions of chondrogenic markers increased compared to control (n = 5) (p-value was obtained from student’s t-test).
Fig. 3. mRNA expression of PKR in ATDC5 cells under hydrostatic pressure measured by real-time PCR.
Graphs show the fold change of PKR-mRNA expression relative to RPL13a mRNA normalized to the control mean. The bars represent the mean ± standard error. mRNA expression of PKR increased compared to control (n = 5) (p-value was obtained student’s t-test).
