Figures & data
Fig. 1. Phenotype of the mpkB∆ strain of A. nidulans.
Notes: (A) Colony growth of the wild-type (left) and mpkB∆ strains (right) on CD plates. (B) Colony diameter of the wild-type and mpkB∆ strains on CD plates.
Fig. 2. Sensitivities of the wild-type and mpkB∆ strains to micafungin and CFW.
Notes: (A) Growth rate after 5 d on CD medium containing micafungin was calculated relative to that of each strain on inhibitor-free CD medium. (B) Growth rate after 5 d on CD medium containing CFW was calculated relative to that of each strain on CFW-free CD medium. Error bars represent the standard error of the mean (n = 3).
Fig. 3. Transcription of cell wall-related genes in the wild-type and mpkB∆ strains after treatment with 0.1 μg/mL of micafungin.
Notes: Levels of transcription of the indicated genes were determined by quantitative RT-PCR using total RNA and the gene-specific primers reported previously by Fujioka et al.Citation5) (Table S1). The transcription of histone H2B gene tended to be reduced in the mpkB∆ strain even in the absence of micafungin (approximately 80% of that of the wild-type strain). According to the differences of the expression levels of histone H2B gene in each strain, the transcription of each gene was normalized. The value shown is the normalized expression level relative to that of the histone H2B gene in each strain. Error bars represent the standard deviations (n ≥ 3). *, Value significantly (p < 0.01) different from that of the wild-type strain.
Fig. 4. Colony growth of the mpkA∆, mpkB∆, CmpkB (conditional-mpkB), and CmpkB-mpkA∆ (CmpkB with mpkA∆) strains on YPD medium (mpkB-repressing condition for CmpkB and CmpkB-mpkA∆ strains) for 5 d.
Fig. 5. Colony diameter of the mpkA∆, mpkB∆, CmpkB (conditional-mpkB), and CmpkB-mpkA∆ (CmpkB with mpkA∆) strains on CDTF medium (mpkB-inducing condition for CmpkB and CmpkB-mpkA∆ strains) and YPD medium (mpkB-repressing condition for CmpkB and CmpkB-mpkA∆ strains) for 5 d.
Fig. 6. Composition of carbohydrate of each cell wall fraction.
Notes: The wild-type, mpkA∆, and mpkB∆ strains were cultured in CD liquid medium at 37 °C for 24 h. Monosaccharide compositions of the (A) hot-water-soluble (HW), (B) alkali-soluble 1 (AS1), (C) alkali-soluble 2 (AS2), and (D) alkali-insoluble (AI) cell wall fractions are expressed as percentages of the total dry weight of each fraction. Error bars represent the standard deviations (n ≥ 3). Glc, glucose; Gal, galactose; Man, mannose; GlcN, glucosamine.
