Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-d-glucitol

Figures & data

Fig. 1. l-sorbose and 1,5-anhydro-d-glucitol.

Table 1. Substrate specificity of purified recombinant sorbose dehydrogenase.

Substrate	Relative activity (%)
l-sorbose	100
1,5-AG	79
d-galactose	1.4
d-glucose	1.0
d-fructose	0.9
d-cellobiose	0.7
2-deoxy-d-glucose	0.4
methyl-β-d-glucopyranoside	0.3
3-o-methyl-d-glucose	0.2
d-glucose-6-phosphate	0.3
d-mannose	0.1
d-sucrose	0.1
d-arabinose	0.1
d-raffinose	0.1
arabitol	0
d-xylose	0
glycerol	0

Notes: Enzyme activity was measured for various substrates (final concentration 333 mM) under standard conditions. Substrate concentration was determined at five times the concentration of the K_m value of sorbose.

Fig. 2. Calibration curve of 1,5-anhydro-d-glucitol.

Note: Enzyme activities were measured under standard conditions at various concentrations of 1,5-anhydro-d-glucitol.