Identification and analysis of the resorcinomycin biosynthetic gene cluster

Figures & data

Fig. 1. Structures of resorcinomycin (1), pheganomycins (3), and their corresponding N-terminal nonproteinogenic amino acids (2 and 4).

Fig. 2. The resorcinomycin biosynthetic gene cluster identified in Streptoverticillium roseoverticillatum.

Note: A schematic resorcinomycin biosynthetic gene cluster (A) and the predicted functions of the genes (B) are shown.

Fig. 3. Disruption of the res5 gene.

Note: Electrophoresis of the PCR-amplified products with genomic DNA of the wild type (1) and Δres5 mutant (2) as templates (A) and a schematic illustration of the amplified regions (B) are shown.

Fig. 4. LC-ESI-MS analysis of products accumulated in the culture broth of the res5 disruptant and S. lividans carrying the res gene cluster.

Notes: Selected ion chromatograms ([M + H]⁺ = 325 and 268) of authentic standard of 1 (A), culture broth of the wild type (B), culture broth of the res5 disruptant (C), 2 prepared from 1 by acid hydrolysis (D), and culture broth of S. lividans harboring the res gene cluster (E) are shown. In (B), (C), and (E), spectra of the detected compounds are also shown. Ions with asterisk in (C) are unrelated to resorcinomycin and also observed in the wild type.