Figures & data
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Fig. 1. Separation of N-Ac-(R)- and (S)-β-Phe acylase activities using a Phenyl Sepharose column.
![Fig. 1. Separation of N-Ac-(R)- and (S)-β-Phe acylase activities using a Phenyl Sepharose column.](/cms/asset/24a26743-3ac3-4082-995d-c4b719d78716/tbbb_a_1072458_f0001_b.gif)
Fig. 2. SDS-PAGE analysis of the purified (A) N-Ac-(R)-β-Phe acylase and (B) N-Ac-(S)-β-Phe acylase.
![Fig. 2. SDS-PAGE analysis of the purified (A) N-Ac-(R)-β-Phe acylase and (B) N-Ac-(S)-β-Phe acylase.](/cms/asset/bd672ff2-7ae9-4b5c-8526-a6aa55aed138/tbbb_a_1072458_f0002_b.gif)
Table 1. Purification of N-Ac-(R)-β-Phe acylase and N-Ac-(S)-β-Phe acylase.
Fig. 3. Enantioselectivity of purified acylases.
![Fig. 3. Enantioselectivity of purified acylases.](/cms/asset/b2cee79d-b0b4-42ac-9658-bcc94e44b7f7/tbbb_a_1072458_f0003_b.gif)
Fig. 4. Genetic organizations around the gene encoding (A) N-Ac-(R)-β-Phe acylase and (B) N-Ac-(S)-β-Phe acylase.
![Fig. 4. Genetic organizations around the gene encoding (A) N-Ac-(R)-β-Phe acylase and (B) N-Ac-(S)-β-Phe acylase.](/cms/asset/1cb33847-1b11-48de-a891-6c3b3d334ceb/tbbb_a_1072458_f0004_b.gif)
Table 2. Location and properties of sequenced genes and proteins predicted to be encoded by those genes.
Fig. 5. Optimum pH (A) and temperature (B) of N-Ac-(R)-β-Phe acylase and N-Ac-(S)-β-Phe acylase.
![Fig. 5. Optimum pH (A) and temperature (B) of N-Ac-(R)-β-Phe acylase and N-Ac-(S)-β-Phe acylase.](/cms/asset/f6f4c443-e098-464f-8c81-4ca0d0400e19/tbbb_a_1072458_f0005_b.gif)