Figures & data
Fig. 1. JCM5805 activates and increases the cytotoxic activity of splenic NK cells. Splenocytes were cultured for 18 h with or without 10 μg/mL heat-killed JCM5805. After 18 h, cultured cells were stimulated for 4.5 h with a cell activation mixture, and then (A) the rate of NK cells expressing intracellular IFN-γ in total NK cells; (B) the rate of NK cells expressing intracellular granzyme B in total NK cells was determined. (C) NK cells cytotoxicity was determined after 18 h culture with or without 10 μg/mL heat-killed JCM5805. Statistical comparisons were performed using the Student’s t-test. Significant differences were compared to the control group, *p < 0.05.
Fig. 2. NK cells cytotoxicity induced by co-culture with BM-DC stimulated by JCM5805. BM-DC were cultured for 8 h with or without 10 μg/mL heat-killed JCM5805 and then co-cultured for 12 h with NK cells isolated from C57BL/6 J spleens using negative selection by magnetic cell separation. After 12 h co-culturing, YAC-1 cells, a target of NK cells, were added and the rate of dead YAC-1 cells was analyzed by flow cytometry. Statistical comparisons were performed using the Student’s t-test. Significant differences were compared to the control group, *p < 0.05.
Fig. 3. Ex vivo effect of the oral administration of JCM5805 on splenic NK cells. Mice (n = 8) were fed with or without heat-killed JCM5805 for 2 weeks. Then, mice were sacrificed and splenocytes were prepared from the collected spleens. Then the cytotoxicity of splenic NK cells was analyzed. Statistical comparisons were performed using the Student’s t-test. Significant differences were compared to the control group, *p < 0.05. Line indicates the mean cytotoxicity %.
