Figures & data
Fig. 1. Transfer frequency of plasmids according to cell density.
Notes: P. putida SM1443 cells were used as donors and P. putida KT2440RG cells as recipients. The OD600 values of donor and recipient cells were adjusted using LB [donor: recipient ratios, 2 × 100: 2 × 101 (1), 2 × 10−1: 2 × 100 (2), 2 × 10−2: 2 × 10−1 (3), 2 × 10−3: 2 × 10−2 (4), 2 × 10−4: 2 × 10−3 (5)] before mixing; the identical ratio of donor to recipient cells (1:10) were used in each cell density. Mixtures were incubated in microtubes (liquid mating; A) or on a solid surface (filter mating; B) for 3 h at 30 °C. Bars show the mean transfer frequencies (transconjugants/donor) calculated from triplicate spots of serially diluted mixtures. Filled diamonds show the frequencies calculated from each spot. Open triangles indicate the numbers of donor cells after mating. All experiments were performed at least twice.
Supplemental material
