A short-term zinc-deficient diet decreases bone formation through down-regulated BMP2 in rat bone

Figures & data

Table 1. Effects of short-term zinc deficiency on body weight, serum mineral levels, and markers of bone metabolism.

	PF	ZD
Body weight (g)
Initial	83.46 ± 1.14	83.37 ± 1.05
Final	110.92 ± 0.81	108.44 ± 1.29
Serum
Zinc (μM)	20.60 ± 0.67	8.57 ± 0.34*
Calcium (mM)	2.65 ± 0.05	2.63 ± 0.09
Osteocalcin (ng/ml)	137.52 ± 12.83	97.22 ± 7.81*
CTx (ng/ml)	135.22 ± 10.33	140.10 ± 7.22

Notes:

Values are expressed as means ± SEM for each group of eight rats.

* Significant differences from the PF group at p < 0.05.

Table 2. Effect of short-term dietary zinc deficiency on the gene expression levels of osteoblastogenesis-related factors and of osteoblast-specific proteins in the femur.

	PF	ZD
BMP2	1.00 ± 0.04	0.57 ± 0.03*
Runx2	1.00 ± 0.03	0.75 ± 0.02*
Osterix	1.00 ± 0.06	0.51 ± 0.02*
ALP	1.00 ± 0.04	0.52 ± 0.01*
Osteocalcin	1.00 ± 0.04	0.71 ± 0.03*
Col1a1	1.00 ± 0.05	0.59 ± 0.04*

Notes:

The mRNA expression of BMP2, Runx2, Osterix, ALP, Osteocalcin, and Col1a1 was determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM (n = 8).

* Significant differences from the PF group at p < 0.05.

Table 3. Effect of short-term dietary zinc deficiency on the gene expression levels of zinc transporters and metallothioneins in the femur.

	PF	ZD
Zip1	1.00 ± 0.03	1.31 ± 0.04*
Zip13	1.00 ± 0.03	1.38 ± 0.03*
Zip14	1.00 ± 0.03	1.44 ± 0.03*
ZnT5	1.00 ± 0.04	0.72 ± 0.02*
ZnT7	1.00 ± 0.03	0.98 ± 0.02
Mt1a	1.00 ± 0.06	0.53 ± 0.02*
Mt2A	1.00 ± 0.07	0.43 ± 0.02*

Notes:

The mRNA expression of Zip1, Zip13, Zip14, ZnT5, ZnT7, Mt1a, and Mt2A was determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM (n = 8).

* Significant differences from the PF group at p < 0.05.