Development of enzyme technology for Aspergillus oryzae, A. sojae, and A. luchuensis, the national microorganisms of Japan

Figures & data

Table 1. Characteristic glycosidases obtained from A. oryzae, A. luchuensis, and A. tubingensis.

Published year	Name of enzyme (Action and use)	(Enzyme Nomenclature)	Author	Reference
1894	Takadiastase (Human digestant)		Takamine J.	7
1949	γ-Amylase (Glucoamylase)	(EC 3.2.1.3)	Kitahara K.	11
1951	α-Amylase (Taka-amylase A)	(EC 3.2.1.1)	Akabori S.	9, 10
1981	α-1,2-Mannnosidase	(EC 3.2.1.113)	Ichishima E.	39

Table 2. Characteristic chemicals obtained from A. oryzae.

Published year	Name of chemicals	Properties	Investigators	Reference
1924	Kojic acid^*	Antibiotic substance	Yabuta T.	96
2004	Ethyl-α-glucoside	Skin dermatitis	Hirotsune M.	59

^* 5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one

Table 3. Characteristic endoribonuclease and endonuclease obtained from A. oryzae.

Published year	Name of enzyme (Action and use)	(Enzyme Nomenclature)	Investigator	Reference
1957	Ribonuclease T1 (guanyloribonuclease, RNase T1)	(EC 3.1.27.3)	Sato K, Egami F.	16
1966	Aspergillus nuclease S1 (single-stranded nucleate endonuclease)	(EC 3.1.30.1)	Ando T.	17

Table 4. Characteristic proteolytic enzymes obtained from A. oryzae, A. sojae, A. luchuensis, and A. tubingensis.

Published year	Name of enzyme	(Enzyme Nomenclature)	Investigator	Reference
1956	Aspergillopepsin I (Aspergillopeptidase A)	(EC 3.4.23.18)	Yoshida F.	24
1972	Serine carboxypeptidase (Acid carboxypeptidase)	(EC 3.4.16.5)	Ichishima E.	32
1972	Deuterolysin	(EC 3.4.24.39)	Sekine H.	19
1972	Orizin (Aspergillopeptidase B)	(EC 3.4.21.63)	Subramanian AR.	74
2003	Aorsin	(EC 3.4.21.-)	Lee BR.	31

Fig. 1. Proposed structures of the new oligosaccharides (Man₁₀GlcNAc₂³⁷⁾ [A] and Man₁₁GlcNAc₂³⁸⁾ [B]) of Aspergillus serine carboxypeptidase.

Notes: The structures of Man₁₀GlcNAc₂ and Man₁₁GlcNAc₂ were determined as Manα1→2Manα→6(Manα1→2Manα1→3)Manα1→6(Manα1→2Manα1→2Manα1→2Manα1→3)Manβ1→4GlcNAcβ1→4GlcNAc³⁷⁾ and Man₁₁GlcNAc₂, Manα1→2Manα1→6(Manα1→2Manα1→3)Manα1→6(Manα1→2Manα1→2Manα1→2Manα1→2Manα1→3)Manβ1→4GlcNAcβ1→4GlcNAc³⁸⁾, respectively.

Chiba Y, Yamagata Y, Nakajima T, et al. A new high-mannose type N-linked oligosaccharide from Aspergillus carboxypeptidase. Biosci. Biotechnol. Biochem. 1992;56:1371–1372.10.1271/bbb.56.1371

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Chiba Y, Yamagata Y, Iijima S, et al. The carbohydrate moiety of the acid carboxypeptidase from Aspergillus saitoi. Curr. Microbiol. 1993;27:281–288.

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