Figures & data
Table 1. Used antibodies for immunohistochemistry.
Fig. 1. Histological examination for mammary glands in P19.
Notes: H&E, TUNEL, ORO stain, and immunohistochemistry for perilipin, keratin 18, and F4/80. All examinations were conducted with mammary gland tissue of P19 dams and under 200× magnification.
Fig. 2. Mammary gland involution in corticosterone-injected B6.IL-10−/− mice.
Notes: (A) Number of apoptotic bodies in mammary gland alveolus was calculated. Data were shown as mean ± SEM (n = 6–7), (B) relative TH mRNA expression analysis by quantitative PCR using adrenal gland. Data were shown as mean ± SEM (n = 4–5), (C) serum corticosterone concentrations analysis with ELISA method. Data were shown as mean ± SEM (n = 4), D) Western blot assays for Stat3 and pStat3 using protein from mammary gland tissue, and (E) frequency of macrophage population (F4/80 and CD11b double positive). Representative individual flow cytometry data were selected and are shown.
Fig. 3. Corticosterone-injected dam developed pup alopecia in B6.IL-10−/−.
Notes: (A) Pup alopecia in corticosterone-injected B6.IL-10−/− dams. Data were shown as number of dam with alopecia pup/total dams, (B) incidence analysis of pup alopecia by macroscopic examination, (C) histological examinations in pup skin with H&E and immune stain with F4/80, and (D) growth curve of pups was analyzed. The dot and error bar indicate the mean of pup weight and the 95% confidence interval.
