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Bioscience, Biotechnology, and Biochemistry Volume 81, 2017 - Issue 1
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Cloning and heterologous expression of a novel subgroup of class IV polyhydroxyalkanoate synthase genes from the genus Bacillus

Kouhei MizunoDepartment of Creative Engineering, Kitakyushu National College of Technology, Kitakyushu, JapanCorrespondence[email protected]
,
Takahiro KiharaDepartment of Innovative and Engineered Materials, Tokyo Institute of Technology, Yokohama, Japan
,
Takeharu TsugeDepartment of Innovative and Engineered Materials, Tokyo Institute of Technology, Yokohama, Japan
,
Benjamin R. LundgrenDepartment of Chemistry, College of Environmental Science and Forestry, State University of New York, Syracuse, NY, USA
,
Zaara SarwarDepartment of Chemistry, College of Environmental Science and Forestry, State University of New York, Syracuse, NY, USA
,
Atahualpa PintoDepartment of Chemistry, College of Environmental Science and Forestry, State University of New York, Syracuse, NY, USA
&
Christopher T. NomuraDepartment of Chemistry, College of Environmental Science and Forestry, State University of New York, Syracuse, NY, USA;Center for Applied Microbiology, College of Environmental Science and Forestry, State University of New York, Syracuse, NY, USA;Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan, People's Republic of China
 show all
Pages 194-196 | Received 18 Jun 2016, Accepted 12 Aug 2016, Published online: 09 Sep 2016

Figures & data

Fig. 1. Schematic diagram of pha operons on the genome sequences of B. bataviensis JCM 21706T and two representative species.

Notes: Colored arrows represent pha genes. The numbers show length of spacer sequences (bp). B. bataviensis JCM 21706T had 3 homologs of phaRC on the genome, one of which shown above with their accession numbers was cloned and analyzed in this study. The sspD encodes small-acid-soluble spore protein.
Fig. 1. Schematic diagram of pha operons on the genome sequences of B. bataviensis JCM 21706T and two representative species.

Fig. 2. 1H-NMR spectra of a PHA sample synthesized from glucose by E. coli JM 109 harboring B. bataviensis phaRC genes (pGEM”PhaRCBbAB).

Note: Signal from water is marked with asterisk.
Fig. 2. 1H-NMR spectra of a PHA sample synthesized from glucose by E. coli JM 109 harboring B. bataviensis phaRC genes (pGEM”PhaRCBbAB).

Fig. 3. Phylogenetic trees of (A) PhaC proteins and (B) PhaR proteins from Bacillus species. B. megaterium,Citation14) B. cereus YB-4,Citation11) INT005,Citation13) and SPVCitation15) marked with asterisks are previously known PhaRC proteins.

Notes: The bold letters represent the PhaRC proteins analyzed in this study. Bootstrap values (expressed as percentages of 1000 replications) >70% are shown at the branch points. Scale bar = 0.1 substitution per amino acid position.
Fig. 3. Phylogenetic trees of (A) PhaC proteins and (B) PhaR proteins from Bacillus species. B. megaterium,Citation14) B. cereus YB-4,Citation11) INT005,Citation13) and SPVCitation15) marked with asterisks are previously known PhaRC proteins.
Supplemental material

TBBB_1230006_Supplementary_Material.pptx

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