Figures & data
Fig. 1. Isoflavone fraction from soybean decreased.
Notes: Renilla luciferase activity. Renilla luciferase activity was measured and normalized by total protein using RLM1 cells stably expressing Renilla luciferase. Luciferase activity was shown as relative activity to control sample. Gex1A (10 ng/mL) was used as positive control. Each value is the mean ± SD of triplicate experiments. Statistical analysis was performed using One-way ANOVA followed by Dunnett’s test. p-values, *p < 0. 05, **p < 0. 01, ***p < 0. 001.
Fig. 2. Isoflavone fraction inhibited mRNA maturation process.
Notes: (A) The bulk mRNA was visualized by Cy3 labeled olio dT45 probe in U2OS cells. Cells were treated with indicated concentration of isoflavone for 24 h. Gex1A (30 ng/mL) was used as positive control. The nuclei were stained with DAPI. (B) Subcellular distribution of mRNA. The signal intensity of the whole cell and that in the nucleus was quantified using ImageJ. The cytoplasmic intensity was calculated by subtracting the nuclear intensity from the whole cellular intensity. Each value is the mean ± SD (n = 8). Statistical analysis was performed using One-way ANOVA followed by Dunnett’s test. p-values, **p < 0. 01, ***p < 0. 001. Scale bar, 10 μm.
Fig. 3. Isoflavone fraction inhibited cell proliferation.
Notes: The effect for the cell proliferation by isoflavone was estimated by MTT assay. Isoflavone fraction was added to U2OS cells for 24 h, and then, MTT regent was added to cell culture media for 4 h. The produced formazan was solubilized with the buffer containing 10 mM NH4Cl and 10% SDS. Absorbance was measured at 600 nm. Relative intensity of control absorbance was calculated as 100%. Each value is the mean ± SD of triplicate experiments. Statistical analysis was performed using One-way ANOVA followed by Dunnett’s test. p-values, **p < 0. 01.
