Figures & data
Fig. 1. Localization of β-xylosidases produced by Aspergillus niger E-1.
Notes: A. niger E-1 was cultivated in XS medium for 48 h, 72 h, 96 h, 120 h, and 144 h. Cell wall-associated fraction (circle), extracellular fraction (square), and intracellular fraction (triangle) were prepared and subjected to β-xylosidase assay. All data are shown by mean values and standard deviations of total activities obtained from mycelia and cultural supernatants prepared from independent three cultures of 50 mL.
Fig. 2. SDS-PAGE of β-xylosidase preparations.
Notes: β-Xylosidase preparations separated by DEAE-Sepharose column chromatography were run on a 7.5% (w/v) denatured polyacrylamide gel. Lane M, protein size marker; lane 1, peak A from extracellular enzyme fraction (15 μg); lane 2, peak B from cell associated enzyme fraction (21 μg); lane 3, Yatalase™ (100 μg).
Fig. 3. Expression profile of β-xylosidase genes from A. niger E-1.
Notes: The expression of the β-xylosidase genes xlsI–V was analyzed by 2% agarose gel electrophoresis after RT-PCR. Panels (A) and (B) show RT-PCR products from 72-h- and 120-h-grown mycelia. White arrows indicate identified RT-PCR products. M, size marker; I, xlsI; II, xlsII; III, xlsIII; IV, xlsIV; V, xlsV. Results of amplification by RT-PCR are summarized in panel (C).
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