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Bioscience, Biotechnology, and Biochemistry Volume 82, 2018 - Issue 1
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Biochemistry & Molecular Biology (Note)

Characterization of cis-4-hydroxy-D-proline dehydrogenase from Sinorhizobium meliloti

Seiya WatanabeDepartment of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Japan;Faculty of Agriculture, Ehime University, Matsuyama, Japan;Center for Marine Environmental Studies, Ehime University, Matsuyama, JapanCorrespondence[email protected]
,
Daichi MorimotoFaculty of Agriculture, Ehime University, Matsuyama, Japan;Graduate School of Agriculture, Kyoto University, Kyoto, Japan
,
Fumiyasu FukumoriFaculty of Food and Nutritional Sciences, Toyo University, Gunma, Japan
&
Yasuo WatanabeDepartment of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Japan;Faculty of Agriculture, Ehime University, Matsuyama, Japan
Pages 110-113 | Received 04 Oct 2017, Accepted 01 Nov 2017, Published online: 01 Dec 2017

Figures & data

Figure 1. Purification and characterization of SmHypO. (a) Library of proline derivatives related to this study. (b) SDS-PAGE analysis of the purified SmHypO recombinant protein (5 μg in a 12% (w/v) gel). (c) Absorption spectra. (d) HPLC analysis of the prosthetic group. Elution profiles of extracts of SmHypO (upper) and the standard mixture (lower). (e) Zymogram staining analysis used the indicated proline derivatives as substrates together with the PMS/INT or PMS/NBT assay system. Purified enzymes (50 μg) were applied on 10% (w/v) non-denaturing PAGE.

Figure 1. Purification and characterization of SmHypO. (a) Library of proline derivatives related to this study. (b) SDS-PAGE analysis of the purified SmHypO recombinant protein (5 μg in a 12% (w/v) gel). (c) Absorption spectra. (d) HPLC analysis of the prosthetic group. Elution profiles of extracts of SmHypO (upper) and the standard mixture (lower). (e) Zymogram staining analysis used the indicated proline derivatives as substrates together with the PMS/INT or PMS/NBT assay system. Purified enzymes (50 μg) were applied on 10% (w/v) non-denaturing PAGE.

Table 1. Kinetic parameters of the SmHypO protein.

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