Branched-chain amino acid (BCAA) supplementation enhances adaptability to exercise training of mice with a muscle-specific defect in the control of BCAA catabolism

Figures & data

Table 1. Body and tissue weights of the control and BDK-mKO mice with BCAA supplementation.

Item	Control	BDK-mKO
	(g)
Body weight	25.4 ± 0.5	26.2 ± 0.3
Tissue	(g/100 g body weight)
Skeletal muscle	0.57 ± 0.02	0.57 ± 0.01
Heart	0.48 ± 0.01	0.50 ± 0.01
Liver	4.12 ± 0.11	4.38 ± 0.11
Epididymal adipose tissue	1.19 ± 0.11	1.35 ± 0.07

Values are means ± SEM (n = 7). Skeletal muscle is a mixture of soleus, gastrocnemius, and plantaris muscles of the left hind-limb. Epididymal adipose tissues (both sides) were collected from the mice and combined.

Figure 1. Endurance capacity of control and BDK-mKO mice. The distance ran to exhaustion before and after 2 weeks’ training with BCAA supplementation. *Significant difference vs. control mice after training (p < 0.05).

Table 2. Plasma amino acid concentrations of the control and BDK-mKO mice with BCAA supplementation.

Amino acid	Control	BDK-mKO
	(μM)
Leucine	163 ± 4	137 ± 7*
Isoleucine	91 ± 2	79 ± 5
Valine	205 ± 7	193 ± 14
BCAAs	463 ± 12	416 ± 25
Alanine	355 ± 23	476 ± 33*
Arginine	67 ± 4	96 ± 9*
Asparagine	64 ± 5	86 ± 4*
Aspartate	24 ± 2	33 ± 3*
Glutamine	636 ± 14	611 ± 19
Glutamate	31 ± 2	43 ± 4*
Methionine	40 ± 2	51 ± 2
Phenylalanine	67 ± 3	67 ± 2
Serine	119 ± 6	131 ± 5
Threonine	117 ± 8	150 ± 8*
Tryptophan	32 ± 2	49 ± 7*
Tyrosine	66 ± 2	82 ± 4*

^* Significant difference vs. control mice (p < 0.05).