Figures & data
Figure 1. Splicing of the HAC1 mRNA by wild-type, V535R, and ∆III Ire1 in S. cerevisiae following treatment with conventional ER-stressing stimuli. (a) KMY1516 (ire1∆) cells or W303-ire1∆ (ire1∆) cells transformed with pRS313-IRE1 or its indicated mutants were stressed by culturing in inositol-depleted SD medium. (b) W303-ire1∆ (ire1∆) cells transformed with pRS313-IRE1 or its indicated mutants were cultured with the indicated chamicals. N: non-stress, D: 10 mM DTT for 30 min, and T: 2 µg/ml tunicamycin for 1 hr.
Figure 2. Splicing of the HAC1 mRNA by wild-type, V535R, and ∆III Ire1 in S. cerevisiae stressed by ethanol. (a) Culturing procedure for ethanol treatment of S. cerevisiae cells. (b) W303-ire1∆ (ire1∆) cells transformed with pRS313-IRE1 or its indicated mutants were stressed by ethanol as shown panel A.
Figure 3. Splicing of the HAC1 mRNA by wild-type and V535R Ire1 in S. cerevisiae cultured with dilute DTT. W303-ire1∆ (ire1∆) cells transformed with pRS313-IRE1 or its V535R mutant were cultured with 1 mM DTT for the indicated durations.
