Reduced CENPU expression inhibits lung adenocarcinoma cell proliferation and migration through PI3K/AKT signaling

Figures & data

Figure 1. CENPU was upregulated in both LAC clinical tissues and cells.

(a-e), Relative level of CENPU in LAC tissues and normal controls collected from 5 cohorts. (f), Relative mRNA level of CENPU in LAC cells and normal lung cells. The data are presented as the means ± SD from three independent experiments. **P < 0.01.

Table 1. The relationship between CENPU expression and clinicopathological features of LAC patients.

	Expression of CENPU
Characteristics	Low	High	P value
Age			0.032*
<60	56	77
≥60	186	165
Gender			0.008*
female	146	117
male	96	125
Pathologic-Stage			0.025*
I + II	202	182
III + IV	40	60
Pathologic-T			0.414
T1 + T2	214	208
T3 + T4	28	34
Pathologic-N			0.001*
N0	179	143
N1 + N2 + N3	63	99
Pathologic-M			0.118
M0	235	228
M1	7	14

Table 2. Univariate and multivariate analysis for overall survival in LAC.

	Univariate analysis			Multivariate analysis
Variables	Pvalue	HR	95%CI	Pvalue	HR	95%CI
CENPU expression（high/low）	0.000*	1.822	1.343–2.473	0.006*	1.546	1.131–2.113
Pathologic-Stage（I + II/III + IV）	0.000*	2.409	1.754–3.310	0.406	1.208	0.773–1.889
Pathologic-T (T1 + T2/T3 + T4)	0.000*	2.240	1.528–3.283	0.006*	1.801	1.180–2.749
Pathologic-M (M0/M1)	0.018*	1.981	1.124–3.490	0.358	1.346	0.715–2.533
Pathologic-N (N0/N1 + N2 + N3)	0.000*	2.476	1.839–3.333	0.000*	1.972	1.377–2.824
Age(＜60/≥60）	0.609	1.091	0.781–1.525
Gender (female/male)	0.367	1.146	0.852–1.541

Figure 2. Kaplan-Meier survival curves of overall survival (OS) based on CENPU expression.

OS rate of patients with low-expression of CENPU were remarkably lower than those of the CENPU-overexpressed patients (log-rank test, P < 0 001)

Figure 3. CENPU silencing inhibited LAC cell proliferation.

(a-b), The expression of CENPU silenced A549 cells were identified by measuring its mRNA and protein level using qRT-PCR and western blot analyses. (c), The OD values of A549 cells were detected by CCK-8 assay. (d), Colony formation assay determined the effect of CENPU knockdown in colony formation ability in A549 cells. The data are presented as the means ± SD from three independent experiments. **P < 0.01.

Figure 4. CENPU silencing repressed the migration and invasion of LAC cells.

(a), Cell migration was determined by a wound-healing assay in the A549 cells transfected with the si-SPOCK1 or si-NC, and the migration rate was quantified. (b), Transwell assays of A549 cells after treatment with si-SPOCK1 or si-NC. The data are presented as the means ± SD from three independent experiments. Scale bar 200 µm. Original magnification: ×200. **P < 0.01.

Figure 5. Downregulation of CENPU suppressed the PI3K/AKT signaling pathway.

(a), Western blot analysis shows changes in the PI3K/AKT signaling pathway components in si-A549 cells. (b), Quantitative analysis of these proteins bands. The data are presented as the means ± SD from three independent experiments. **P < 0.01.