https://orcid.org/0000-0002-0921-2128
Figures & data
Figure 1. Induction of Reg3β protein in enteroids after IL-22 stimulation.
Representative confocal images of immunohistochemistry (Reg3β, green; nucleus, blue) for enteroids treated in the absence of IL-22 (control) and the presence of 10 ng/mL IL-22 [(IL-22 (+)] for 24 h. White arrow heads indicate Reg3β-producing cells. Scale bar is 50 μm.
![Figure 1. Induction of Reg3β protein in enteroids after IL-22 stimulation.Representative confocal images of immunohistochemistry (Reg3β, green; nucleus, blue) for enteroids treated in the absence of IL-22 (control) and the presence of 10 ng/mL IL-22 [(IL-22 (+)] for 24 h. White arrow heads indicate Reg3β-producing cells. Scale bar is 50 μm.](/cms/asset/e604e1f1-c1ac-41bd-b1cf-f87b5e484d89/tbbb_a_1695575_f0001_oc.jpg)
Figure 2. The mRNA levels of marker of intestinal epithelial cells in enteroids after IL-22 stimulation.
The mRNA levels of Itf, ChgA, Dclk1, Sglt1, Lgr5, CD133, and lysozyme in enteroids after IL-22 stimulation (10 ng/mL) for 24 h were measured by qPCR. The mRNA level of Reg3β was normalized with that of β2 microglobuin, the expression level of the control group was set to 1, and that of the IL-22-treated group was relatively expressed. Bars represent means ± SE (n = 4). The comparison of means was performed with Student’s t-test. Asterisks represent a significant difference (p < 0.05).
Figure 3. Immunohistochemical detection of Reg3β and lysozyme in enteroids after IL-22 stimulation.
Representative confocal images of immunohistochemistry (Reg3β, green; lysozyme, red; nucleus, blue) for enteroids treated in the absence of IL-22 (control) and the presence of 10 ng/mL IL-22 [IL-22 (+)] for 24 h. Green and red arrowheads indicate Reg3β-producing cells and Paneth cells, respectively. Yellow arrowheads indicate the overlapping of Reg3β-producing and Paneth cells. Scale bar is 50 μm.
![Figure 3. Immunohistochemical detection of Reg3β and lysozyme in enteroids after IL-22 stimulation.Representative confocal images of immunohistochemistry (Reg3β, green; lysozyme, red; nucleus, blue) for enteroids treated in the absence of IL-22 (control) and the presence of 10 ng/mL IL-22 [IL-22 (+)] for 24 h. Green and red arrowheads indicate Reg3β-producing cells and Paneth cells, respectively. Yellow arrowheads indicate the overlapping of Reg3β-producing and Paneth cells. Scale bar is 50 μm.](/cms/asset/6ec47217-a660-4ea7-92e8-20406b36a442/tbbb_a_1695575_f0003_oc.jpg)
