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Biochemistry & Molecular Biology

Novel method for l-methionine determination using l-methionine decarboxylase and application of the enzyme for l-homocysteine determination

, , , , &
Pages 927-935 | Received 01 Oct 2019, Accepted 28 Dec 2019, Published online: 20 Jan 2020

Figures & data

Figure 1. Scheme of l-methionine determination using MetDC and l-methionine and l-homocysteine determination using MGL.

Figure 1. Scheme of l-methionine determination using MetDC and l-methionine and l-homocysteine determination using MGL.

Figure 2. Calibration curve for l-methionine determination using MetDC.

Figure 2. Calibration curve for l-methionine determination using MetDC.

Figure 3. Correlation between the enzymatic assay and HPLC method for l-methionine.

Figure 3. Correlation between the enzymatic assay and HPLC method for l-methionine.

Figure 4. Effect of blood serum on l-methionine quantification with MetDC.

Figure 4. Effect of blood serum on l-methionine quantification with MetDC.

Figure 5. Elimination of l-methionine from sample solution by MetDC.

Figure 5. Elimination of l-methionine from sample solution by MetDC.

Figure 6. L-Homocysteine determination by MGL after pretreatment of MetDC.

Figure 6. L-Homocysteine determination by MGL after pretreatment of MetDC.

Data availability statement

The data described in this article are openly available in the Open Science Framework at DOI:10.17605/OSF.IO/TPA6U.

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