Symbiotic riboflavin degradation by Microbacterium and Nocardioides bacteria

Figures & data

Table 1. Strains isolated in this study.

Strain	Utilization as carbon source		Predicted strain	Degradation activity* (nmol min^−1 mg^−1 protein)
	Riboflavin	Lumichrome
R13	+	−	Microbacterium paraoxydans	10.9 ± 1.0
R14	+	−	Microbacterium paraoxydans	12.2 ± 2.2
R15	+	−	Microbacterium paraoxydans	13.9 ± 3.0
R16	+	−	Microbacterium paraoxydans	9.3 ± 1.8
R23	+	−	Microbacterium ginsengiterrae	7.3 ± 2.7
R27	+	−	Microbacterium paraoxydans	13.0 ± 1.0
L13	−	+	Nocardioides nitrophenolicus	14.7 ± 6.1
L14	−	+	Nocardioides nitrophenolicus	7.7 ± 3.0
L16	−	+	Nocardioides nitrophenolicus	6.3 ± 2.0

*Pellet fractions of cell-free extracts.

Figure 1. Identification of bacterial isolates that degraded riboflavin and lumichrome.

Figure 2. Riboflavin and lumichrome degradation assays of M. paraoxydans R16 and N. nitrophenolicus L16 on agar plates.

Figure 3. Riboflavin degradation product generated by M. paraoxydans R16 identified using HPLC.

Figure 4. Riboflavin and lumichrome degradation by M. paraoxydans R16 and N. nitrophenolicus L16.

Data availability statement

The data described in this article are openly available in the Open Science Framework at DOI:10.17605/OSF.IO/TPA6U.