Pongamia pinnata influences the circadian variations of liver marker enzymes in hyperammonemic rats

Abstract

In this study, the influences of Pongamia pinnata, an indigenous medicinal plant used in Ayurvedic and traditional Medicine in India, on the circadian variations of liver marker enzymes in ammonium chloride (AC) induced hyperammonemic rats were studied. Experimental rats (160 – 180 g) were divided into control, AC (daily i.p. injection of AC (100 mg kg⁻¹ body weight)) treated, AC + P. pinnata ethanolic leaf extract (PPEt) (300 mg kg⁻¹ body weight) treated and PPEt treated groups. Temporal characteristics (acrophase, amplitude and mesor) of liver marker enzymes; alkaline phosphatase (ALP), aspartate and alanine transaminases (ALT and AST) and γ-glutamyl transferase (GGT) were analyzed. Elevated liver marker enzymes (increased mesor and delayed acrophase of AST, ALT, ALP and GGT) were found in hyperammonemic rats. Administration of PPEt significantly alters these changes. Variations in acrophase, amplitude and r values were also found in control and experimental rats. The detectable circadian rhythms of hepatic marker enzymes and their alterations during AC/PPEt treatments, in the present study, deserve further investigation for the diagnosis and for the therapeutic efficacy of hyperammonemia.

Keywords:

• Hyperammonemia
• circadian
• liver marker enzymes
• Pongamia pinnata