Haloperidol, but not olanzapine, may affect expression of PER1 and CRY1 genes in human glioblastoma cell line

Figures & data

Table 1. Primer sequences of investigated genes for RT-qPCR.

Gene	5′-3′ primer sequences
	Forward	Reverse
CLOCK	TCGACAGGACTGGAAACCTAC	GCTTCCATCTGTCATGATTGC
BMAL1	CCACCAATCCATACACAGAAGC	TCCCTCGGTCACATCCTACG
PER1	GTCCGTCTTCTGCCGTATCA	GTCACATACGGGGTTAGGCG
CRY1	TTGGAAAGGAACGAGACGCA	GCGGTTGTCCACCATTGAGT
GAPDH	AGCCACATCGCTCAGACA	GCCCAATACGACCAAATCC

Notes: CLOCK – circadian locomotor output cycles kaput; BMAL1 – brain and muscle ARNTL-like protein 1; PER – Period; CRY – Cryptochrome; GAPDH –glyceraldehyde 3-phosphate dehydrogenase.

Table 2. Mean values representing the effect of two concentrations (5 and 20 μM) of haloperidol and olanzapine on fold change in expression of the investigated clock genes in glioblastoma cell cultures.

	Haloperidol		Olanzapine
	5 μM	20 μM	5 μM	20 μM
CLOCK	1.49 (1.04–2.13)	0.41 (0.13–1.31)	1.27 (0.76–2.09)	0.53 (0.13–2.16)
BMAL1	1.32 (1.02–1.71)	0.64 (0.40–1.04)	1.01 (0.60–1.7)	0.58 (0.24–1.4)
PER1	1.36 (1.23–1.51)*	0.50 (0.21–1.23)	1.04 (0.85–1.29)	0.80 (0.52–1.23)
CRY1	0.05 (0.01–0.82)**	0.54 (0.24–1.2)	0.81 (0.45–1.46)	0.55 (0.26–1.15)

Note: CLOCK – circadian locomotor output cycles kaput; BMAL1 – brain and muscle ARNTL-like protein 1; PER1 – Period 1; CRY1 – Cryptochrome 1. Presented with normalized values, defined as (Livak & Schmittgen 2001).

^* p<0.05

^** p<0.001.

Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25:402–408.10.1006/meth.2001.1262

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