Sorting of micron-sized particles using holographic optical Raman tweezers in aqueous medium

ABSTRACT

We have constructed a holographic optical tweezers system combined with Raman spectroscopy to sort trapped particles. Our software automatically moves the trapped objects to the measurement positions to obtain individual Raman signals from multiple trapped particles. We performed the sorting by comparing their spectra with the previously measured training dataset using the correlation coefficients. We used yeast cells and polystyrene beads as test particles. This study aims to show that biological particles can be separated using single cell analysis with combined holographic optical tweezers and Raman spectroscopy system.

KEYWORDS:

• Holographic optical tweezers
• Raman spectroscopy
• multivariate statistical analysis
• particle sorting

Disclosure statement

No potential conflict of interest was reported by the authors.

ORCID

Uğur Parlatan  http://orcid.org/0000-0001-7106-5993

Additional information

Funding

Gunay Basar and Ugur Parlatan are grateful to the support offered by Istanbul Technical University Scientific Research Fund (Project Nos: 38202 and 37851).