Abstract
The effect of rutin on ovarian ischemia-reperfusion (I/R) injury was investigated in this experimental study. Eighteen Wistar albino female rats were divided into three groups as follows: I/R group (IRG; n = 6), 50 mg/kg rutin + I/R group (RG; n = 6), and a healthy control group scheduled for a sham operation (SG; n = 6). 2 h of ischemia and following 2 h of reperfusion were created in the IRG and RG by using a torsion model involving atraumatic vascular clips. Rutin, a flavonoid glycoside, was injected intraperitoneally at the dose of 50 mg/kg to RG group 1 h before reperfusion. Then, rats were euthanized and their ovaries were removed for biochemical and histopathological examination and also assessment of the gene expressions. IRG group had a significant increase in malondialdehyde (MDA) levels, in the expressions of tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β), and also in the activity of cyclooxygenase 2 (COX-2) unlike the significant decrease in total glutathione (tGSH) levels and the activity of COX-1 when compared to the SG group. However, rutin significantly decreased MDA levels, the expressions of TNF-α and IL-1β, and also the activity of COX-2 while it increased significantly tGSH levels and the activity of COX-1 in the RG group in comparison with the IRG group. Rutin ameliorated the I/R-induced ovarian injury in rats via its possible antioxidative and anti-inflammatory effects.
Chinese abstract
在本试验性研究中调查了芸香苷在卵巢缺血再灌注(I/R)损伤中的作用。18只Wistar白化雌性大鼠分为如下3组:I/R组(IRG, n=6), 50mg/kg芸香苷+I/R组(RG, n=6), 假手术的健康对照组(SG, n=6)。通过使用扭转模型, 包括无损伤作用的血管夹, 在IRG组和RG组进行了2h的缺血以及之后2h的再灌注。芸香苷, 一种黄酮醇配糖体, 再灌注前1h以50mg/kg 的剂量腹腔注射到RG组。然后, 老鼠被安乐死, 取下卵巢组织做生化、组织病理学检查以及评估基因表达。与SG组相比, IRG组丙二醛(MDA)水平、肿瘤坏死因子-a(TNF-a)和白细胞介素-1b(IL-1b)的表达以及环氧合酶-2(COX-2)活性明显升高, 总谷胱甘肽(tGSH)水平以及环氧合酶-1(COX-1)的活性明显下降。然而, 与IRG组相比, 在RG组中, 芸香苷明显增加tGSH水平与COX-1活性的同时明显降低MDA水平、TNF-a和IL-1b的表达以及COX-2的活性。芸香苷通过其可能的抗氧化和抗炎作用改善I/R导致的卵巢损伤。
Disclosure statement
The authors report no conflicts of interest.