Association of IL-10, IL-18, and IL-33 genetic polymorphisms with recurrent pregnancy loss risk in Iranian women

Abstract

Recurrent pregnancy loss (RPL) is a heterogeneous disease with three or more consecutive abortions before 20 weeks of pregnancy. Recently, inflammatory factors such as interleukins (IL) have been found to be a significant factor in the RPL. The objective of this study was to investigate the association between RPL and IL-10 (rs1800896), IL-18 (rs1946518) and IL-33 (rs1929992) genes polymorphisms in Iranian women. The study participants consisted of 300 women with RPL and the control group comprised of 300 healthy women with successful delivery. Genomic DNA was extracted from peripheral blood, and genotyping was performed by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). There were no significant differences in the frequencies of genotype and allele in IL-10 gene polymorphism (rs1800896) between patients and control group (p > .005). In contrast, there were significant differences in the frequencies of CC genotype in IL‐18 gene polymorphism (rs1946518) between patients and the control groups (p = .004; OR =0.990; 95% CI: 0.320–8.855). Also, there were significant differences in the frequencies of GA genotype in IL‐33 gene polymorphism (rs1929992) between patients and the control groups (p = .001; OR =0.955; 95% CI: 0.239–9.807). Present study showed that the rs1800896 polymorphism (IL-10) might not play role in RPL in the Iranian population; whereas rs1946518 (IL-18) and rs1929992 (IL-33) polymorphisms may be associated with the risk of RPL in the Iranian women.

摘要

复发性流产（RPL）是一种异质性的疾病, 在妊娠20周之前连续流产三次或三次以上。最近, 炎症因子如白介素（IL）已被发现是RPL的一个重要因素。本研究旨在探讨伊朗女性的PRL与IL-10 (rs1800896), IL-18 (rs1946518) 和 IL-33 (rs1929992) 基因多态性的关系。研究的参与者包括300名PRL女性和300名成功分娩的健康女性作为对照组。从外周血中提取基因组DNA, 基因分析采用聚合酶链反应（PCR）随后进行限制性片段长度多态性（RFLP）。IL-10基因多态性位点(rs1800896)的基因型和等位基因频率在病例组和对照组之间无显著差异(p>.005)。相反, IL-18基因多态性位点(rs1946518)的CC基因型频率在病例组和对照组相比有显著差异(p=.004; OR=0.990; 95% CI: 0.320–8.855)。并且, IL-33基因多态性位点(rs1929992)的GA基因型频率在病例组与对照组相比有显著差异(p=.001; OR =0.955; 95% CI: 0.239–9.807)。本研究表明, rs1800896多态性（IL-10）在伊朗人群中可能与RPL不相关, 而rs1946518 (IL-18) 和rs1929992 (IL-33)多态性可能与伊朗女性RPL风险增高相关。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• IL-10
• IL-18
• IL-33
• polymorphism
• recurrent pregnancy loss

Acknowledgments

The authors thank the participants for being involved in this study.

Disclosure statement

No potential conflict of interest was reported by the authors.