Resveratrol improves ovarian state by inhibiting apoptosis of granulosa cells

Figures & data

Table 1. Primer sequence and amplification product size.

Gene name	Primer sequence	Amplification product (bp)
ACTB	Forward 5′-CTTCGCGGGCGACGAT-3′ Reverse 5′-CCACATAGGAATCCTTCTGACC-3′	104
BCL-2	Forward 5′-ATAACGGAGGCTGGGTAGGT-3′ Reverse 5′-CCACATAGGAATCCTTCTGACC-3′	127
BAX	Forward 5′-CAGAGGCGGGGGATGATTG-3′ Reverse 5′-TGTCCAGCCCATGATGGTTC-3′	198
Caspase 9	Forward 5′-AGCCAGAGGTTCTCAGACCA-3′ Reverse 5′-TCTTGGCAGTCAGGTCGTTC-3′	282
Caspase 8	Forward 5′-CACCTTGTGTCTGAGCTGGT-3′ Reverse 5′-GGTTCTTGCTTCCTTTGCGG-3′	319
Caspase 3	Forward 5′-TTGTGAGGCGGTTGTAGAAGAGTTT-3′ Reverse 5′-TTTATTAACGAAAACCAGAGCGCC-3′	157
TGF-β1	Forward 5′-CTGTCCAACATGATCGTGCG-3′ Reverse 5′-ACACAGAGATCCGCAGTCCT-3′	193
GDF 9	Forward 5′-GTCATTTCCGGCAAGGTTGG-3′ Reverse 5′-TGCAAAGCTCTGGAGTCTGG-3′	70
BMP 15	Forward 5′-GAAGAATCCCCTGGCGAACA-3′ Reverse 5′-CCGGTACAACTCCAGCATGT −3′	78

Figure 1. The Effect of RES on apoptosis of human ovarian granulosa cells under oxidative stress by flow cytometry.

Figure 2. Effects of different concentrations of RES on the apoptosis rate of oxidized human ovarian granulosa cells.

Figure 3. Effects of RES on apoptosis and proliferation related protein expression of hGCs.

Figure 4. Effects of RES on apoptosis and proliferation related mRNA expression of hGCs.