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Research Article

Protective effect of wuzibushen recipe on follicular development via regulating androgen receptor in polycystic ovary syndrome model rats

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Article: 2190808 | Received 05 Jan 2023, Accepted 09 Mar 2023, Published online: 24 Mar 2023

Figures & data

Table 1. qRT-PCR primers

Figure 1. WZBS recipe alleviated the ovarian and uterine injuries of the LE-induced PCOS rats. (A) WZBS recipe significantly increased the weight of bilateral ovaries but had no effect on the weight of uterus in the PCOS rats. (B) WZBS recipe improved the ovarian and uterine morphology of the PCOS rats. *p < 0.05 and **p < 0.01 vs. Control. Data were presented as mean ± SD. n = 8.

Figure 1. WZBS recipe alleviated the ovarian and uterine injuries of the LE-induced PCOS rats. (A) WZBS recipe significantly increased the weight of bilateral ovaries but had no effect on the weight of uterus in the PCOS rats. (B) WZBS recipe improved the ovarian and uterine morphology of the PCOS rats. *p < 0.05 and **p < 0.01 vs. Control. Data were presented as mean ± SD. n = 8.

Figure 2. WZBS recipe normalized the estrous cycle of LE-induced PCOS rats. All the pictures were original magnification ×200. n = 3.

Figure 2. WZBS recipe normalized the estrous cycle of LE-induced PCOS rats. All the pictures were original magnification ×200. n = 3.

Figure 3. WZBS recipe ameliorated ovary histopathological injuries of PCOS rats. (A) The representative images of ovarian tissue of rats stained with HE (×40, ×200, ×400, respectively). (B) The representative images of ovarian tissue of rats stained with oil red O (×200, ×400, respectively). n = 3.

Figure 3. WZBS recipe ameliorated ovary histopathological injuries of PCOS rats. (A) The representative images of ovarian tissue of rats stained with HE (×40, ×200, ×400, respectively). (B) The representative images of ovarian tissue of rats stained with oil red O (×200, ×400, respectively). n = 3.

Figure 4. WZBS recipe increased the number of mature follicles and corpora lutea in LE-induced PCOS rats. *p < 0.05 and **p < 0.01 vs. Control, #p < 0.05 and ##p < 0.01 vs. PCOS. Data were presented as mean ± SD. n = 3.

Figure 4. WZBS recipe increased the number of mature follicles and corpora lutea in LE-induced PCOS rats. *p < 0.05 and **p < 0.01 vs. Control, #p < 0.05 and ##p < 0.01 vs. PCOS. Data were presented as mean ± SD. n = 3.

Figure 5. WZBS recipe affected the serum content of T and SHBG, the expression of AR and CX43 in LE-induced PCOS rats. (A) WZBS recipe decreased serum T content and increased serum SHBG content in the PCOS rats. (B) WZBS recipe decreased AR mRNA expression and increased CX43 mRNA expression in the PCOS rats. (C) WZBS recipe decreased AR protein expression and increased CX43 protein expression in the PCOS rats.*p < 0.05 and **p < 0.01 vs. Control, #p < 0.05 and ##p < 0.01 vs. PCOS. Data were presented as mean ± SD. n = 3.

Figure 5. WZBS recipe affected the serum content of T and SHBG, the expression of AR and CX43 in LE-induced PCOS rats. (A) WZBS recipe decreased serum T content and increased serum SHBG content in the PCOS rats. (B) WZBS recipe decreased AR mRNA expression and increased CX43 mRNA expression in the PCOS rats. (C) WZBS recipe decreased AR protein expression and increased CX43 protein expression in the PCOS rats.*p < 0.05 and **p < 0.01 vs. Control, #p < 0.05 and ##p < 0.01 vs. PCOS. Data were presented as mean ± SD. n = 3.

Data availability statement

All the data are contained in the manuscript.