Evaluation of new biomarkers in stage III and IV endometriosis

Figures & data

Graph 1. ROC curve of ANXA5 values in differentiating patients with endometriosis.

Graph 2. ROC curve of sICAM-1 values in differentiating patients with endometriosis.

Graph 3. ROC curve of IL-6 values in differentiating patients with endometriosis.

Graph 4. ROC curve of TNF- α values in differentiating endometriosis patients.

Graph 5. ROC curve of sVCAM-1 values in differentiating patients with endometriosis.

Graph 6. ROC curve of VEGF values in differentiating patients with endometriosis.

Graph 7. ROC curve of Ca 125 values in differentiating endometriosis patients.

Table 1. Comparison of preoperative and postoperative biomarker values of patients in the endometriosis group (n = 30) and control group (n = 49).

		Mean ± SD Median (25th ,75th percentile)	Mean ± SD Median (25th ,75th percentile)	p* value
		Preoperative	Postoperative
ANXA5	Endometriosis	0.19 ± 0.89 0.01 (0.01–0.01)	0.54 ± 1.60 0.02 (0.01–0.025)	0.001
	Control	1.73 ± 5.48 0.01 (0.01–0.015)	1.32 ± 4.33 0.02 (0.01–0.020)	0.127
	p** value	0.097	0.932
sICAM-1	Endometriosis	1277.75 ± 545.52 1229.5 (897.5–1589.7)	1288.21 ± 508.26 1240.5 (962.9–1601.7)	0.975
	Control	1477.17 ± 517.48 1439.1 (1122.2–1850.7)	1404.21 ± 384.24 1388.3 (1158.2–1706.1)	0.615
	p** value	0.087	0.241
IL-6	Endometriosis	5.14 ± 8.21 1.51 (0.10–7.07)	0.93 ± 0.19 0.90 (0.90–0.90)	0.064
	Control	1.97 ± 2.35 0.71 (0.10–3.20)	0.90 ± 0.00 0.90 ((0.90–0.90)	0.028
	p** value	0.310	0.201
TNF-α	Endometriosis	0.18 ± 0.47 0.05 (0.05–0.05)	0.56 ± 1.34 0.10 (0.01–0.41)	0.047
	Control	0.37 ± 0.94 0.05 (0.05–0.25)	0.31 ± 0.50 0.10 (0.05–0.31)	0.196
	p** value	0.400	0.340
sVCAM-1	Endometriosis	975.00 ± 300.43 936.61 (822.50–1059.77)	910.87 ± 431.67 819.55 (696.51–998.90)	0.020
	Kontrol	906.23 ± 209.87 853.39 (759.51–1055.01)	858.47 ± 225.48 843.09 (695.13–943.57)	0.081
	p** value	0.186	0.896
VEGF	Endometriosis	151.31 ± 170.32 99.47 (16.19–240.20)	117.87 ± 130.57 73.84 (25.02–174.51	0.491
	Control	170.05 ± 203.27 109.72 (32.25–204.62)	188.82 ± 201.60 131 (45.82–254.05)	0.131
	p** value	0.628	0.117
Ca-125	Endometriosis	201.28 ± 471.73 68.55 (27.07–126.82)	49.41 ± 73.50 24.55 (19.55–33.17)	<0.001
	Control	18.97 ± 7.38 19 (13–23.90)	20.18 ± 7.35 19.40 (13.05–25.75)	0.249
	p** value	<0.001	0.005

*Wilcoxon Test.

**Mann Whitney U test.

Table 2. ROC curves and diagnostic performance of biomarker values in distinguishing patients with endometriosis.

	AUC 95% CI	p	Threshold	Sensitivity 95% CI	Specificity 95% CI	PPV	NPV
ANXA5	0.58 0.45–0.70	0.256	<0.77	0.97 (0.83–0.99)	0.18 (0.10–0.31)	0.42 (0.31–0.54)	0.90 (0.81–0.95)
sICAM-1	0.61 0.48–0.74	0.087	<1286.5	0.60 (0.42–0.75)	0.67 (0.53–0.79)	0.53 (0.41–0.64)	0.73 (0.62–0.82)
IL-6	0.59 0.43–0.70	0.325	>8.14	0.23 (0.12–0.41)	0.100 (0.93–1.00)	0.100 (0.94–0.1.00)	0.68 (0.56–0.78)
TNF-α	0.54 0.41–0.67	0.528	<0.55	0.97 (0.83–0.99)	0.18 (0.10–0.31)	0.42 (0.31–0.54)	0.90 (0.81–0.95)
sVCAM-1	0.59 (0.46–0.72)	0.186	>901.7	0.63 (0.45–0.78)	0.59 (0.45–0.72)	0.49 (0.37–0.60)	0.72 (0.61–0.82)
VEGF	0.53 0.40–0.68	0.628	<23.2	0.30 (0.17–0.48)	0.82 0.69–0.90	0.50 (0.39–0.61)	0.65 (0.54–0.76)
Ca-125	0.86 0.77–0.96	<0.001*	>32.4	0.73 0.55–0.86	0.98 0.89–0.99	0.96 0.88–0.99	0.86 0.76–0.92

Table 3. Diagnostic performance of biomarker combinations in differentiating patients with endometriosis.

	Sensitivity 95% CI	Specificity 95% CI	PPV	NPV
Ca-125& ANXA5	0.73 (0.55–0.86)	0.100 (0.93–1.00)	0.100 (0.94–0.99)	0.86 (0.76–0.92)
Ca-125& sICAM-1	0.43 (0.27–0.61)	0.98 (0.89–0.99)	0.93 (0.84–0.97)	0.74 (0.62–0.83)
Ca-125& IL-6	0.17 (0.07–0.33)	0.100 (0.93–0.100)	0.100 (0.94–0.99)	0.66 (0.54–0.76)
Ca-125& TNF-α	0.70 (0.52–0.83)	0.98 0.89–0.99)	0.95 (0.87–0.99)	0.84 (0.74–0.91
Ca-125& sVCAM-1	0.50 (0.33–0.67)	0.98 (0.89–0.99)	0.94 0.85–0.98)	0.76 (0.65–0.85)
Ca-125& VEGF	0.17 (0.07–0.33)	0.100 (0.93–0.100)	0.100 (0.94–0.99)	0.66 (0.54–0.76)
ANXA5& ICAM-1	0.57 (0.39–0.73)	0.75 (0.62–0.85)	0.59 (0.47–0.69)	0.74 (0.63–0.83)
ANXA5& IL-6	0.20 (0.09–0.37)	0.100 (0.93–0.100)	0.100 (0.94–0.99)	0.67 (0.55–0.77)
ANXA5& TNF-α	0.93 (0.79–0.98)	0.31 (0.19–0.44)	0.45 (0.34–0.57)	0.88 (0.78–0.94)
ANXA5& sVCAM-1	0.63 (0.45–0.78)	0.69 (0.55–0.80)	0.56 (0.44–0.67)	0.75 (0.64–0.84)