Talin1 regulates glucose metabolism and endometrial receptivity via GLUT-4 in patients with polycystic ovary syndrome and insulin resistance

Figures & data

Table 1. Baseline characteristics of patients in two groups.

Parameters	PCOS-IR	NC	P
Age (years)	25.40 ± 5.19	24.80 ± 4.41	0.736
BMI (kg/m^2)	27.06 ± 2.94	21.00 ± 1.76	0.000
Basal FSH (IU/L)	5.33 ± 1.40	5.91 ± 1.52	0.287
Basal LH (IU/L)	10.50(7.90,12.30)	5.50(4.80,5.90)	0.000
LH/FSH	2.11(1.52,2.67)	0.90(0.62,1.10)	0.000
T (nmol/l)	1.28(1.02,1.68)	0.89(0.70,1.03)	0.001
FBG (mmol/L)	5.32 ± 0.40	4.23 ± 0.47	0.000
Fasting insulin (pmol/L)	155.60(106.10,189.50)	54.00(41.00,59.80)	0.000
HOMA-IR	5.01(3.69,6.72)	1.42(1.06,1.81)	0.000

PCOS-IR: polycystic ovary syndrome and insulin resistance; NC: normal controls; BMI: body mass index; FSH: follicle-stimulating hormone; LH: luteinizing hormone; T: testosterone; FBG: fasting blood-glucose; HOMA-IR: homeostatic model assessment of insulin resistance.

Figure 1. Expression and localization of Talin1 and GLUT4 in endometrium of PCOS-IR and control patients. (A) Localization of Talin1 and GLUT4 in the endometrium; (B, C) mRNA and protein levels of Talin1 and GLUT4 in endometrium of PCOS-IR and control patients. **p < 0.01, ***p < 0.001.

Figure 2. Effect of Talin1 on GLUT4 expression. (A, B) mRNA and protein levels were analyzed by RT-qPCR and western blot after overexpression and silencing of Talin1; (C) The interaction between Talin1 and GLUT4 proteins was examined by Co-IP. ***p < 0.001.

Figure 3. The role of Talin1 in endometrium of PCOS-IR was examined in vivo. (A) The estrus cycles in model and control mice; (B) ovarian changes in model and control mice; (C) Fasting blood glucose, fasting insulin, and HOMA-IR were measured in model and control mice; (D) FSH, LH, and T were measured in model and control mice; (E) Expression of Talin1 and GLUT4 in endometrium of PCOS-IR and control mice; (F) Effect of Talin1 on embryo implantation and live birth number in mice. *p < 0.05, **p < 0.01, ***p < 0.001.

Data availability statement

The data supporting the findings in this study are available from the corresponding author on reasonable request.